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Rad51 Rabbit pAb(bs-20297R)-50

ul/100ul/200ul
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  • ￥1180 - 2800
  • Bioss已认证
  • bs-20297R
  • 2025年10月16日
  • 产品信息以Bioss网站为准
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北京博奥森生物技术有限公司
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      50ul/100ul/200ul

    规格：50ul产品价格：￥1180.0
    规格：100ul产品价格：￥1980.0
    规格：200ul产品价格：￥2800.0
    产品编号bs-20297R
    英文名称Rad51 Rabbit pAb
    中文名称Rad51抗体
    英文别名BRCA1/BRCA2 containing complex, subunit 5; BRCC 5; BRCC5; DNA repair protein RAD51 homolog 1; DNA repair protein rhp51; E coli RecA homolog; HGNC:9817; Homolog of E coli RecA; homolog of S cerevisiae RAD51; HRAD51; HsRad51; HsT16930; Rad 51; RAD51 homolog(RecA homolog, E. coli)(S. cerevisiae); RAD51 homolog; RAD51 homolog S. cerevisiae; RAD51 S cerevisiae homolog; RAD51A; RECA; RecA homolog E. coli; RecA like protein; RecA, E. coli, homolog of; recombination protein A.
    产品应用WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=2ug/Test

    Not yet tested in other applications.
    Optimal working dilutions must be determined by the end user.

    交叉反应Human, Mouse, Rat (Pig, Cow, Horse, Rabbit)
    抗体来源Rabbit
    免疫原KLH conjugated synthetic peptide derived from human Rad51
    亚型IgG
    性状Liquid
    纯化方法affinity purified by Protein A
    克隆类型Polyclonal
    理论分子量37 kDa
    浓度1mg/ml
    储存液Preservative: 0.02% Proclin300, Constituents: 1% BSA, 0.01M PBS, pH7.4.
    研究领域

    Epigenetics and Nuclear Signaling > DNA / RNA > DNA Damage & Repair > Homologous Recomb.

    亚细胞定位Nucleus. Cytoplasm. Cytoplasm, perinuclear region. Mitochondrion matrix. Note=Colocalizes with RAD51AP1 and RPA2 to multiple nuclear foci upon induction of DNA damage. DNA damage induces an increase in nuclear levels.
    组织特异性Highly expressed in testis and thymus, followed by small intestine, placenta, colon, pancreas and ovary. Weakly expressed in breast.
    翻译后修饰Phosphorylated. Phosphorylation of Thr-309 by CHEK1 may enhance association with chromatin at sites of DNA damage and promote DNA repair by homologous recombination. Phosphorylation by ABL1 inhibits function.
    相似性Belongs to the RecA family. RAD51 subfamily.
    Contains 1 HhH domain.
    功能Participates in a common DNA damage response pathway associated with the activation of homologous recombination and double-strand break repair. Binds to single and double stranded DNA and exhibits DNA-dependent ATPase activity. Underwinds duplex DNA and forms helical nucleoprotein filaments. Plays a role in regulating mitochondrial DNA copy number under conditions of oxidative stress in the presence of RAD51C and XRCC3.
    保存条件Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
    注意事项This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
    背景资料The protein encoded by this gene is a member of the RAD51 protein family. RAD51 family members are highly similar to bacterial RecA and Saccharomyces cerevisiae Rad51, and are known to be involved in the homologous recombination and repair of DNA. This protein can interact with the ssDNA-binding protein RPA and RAD52, and it is thought to play roles in homologous pairing and strand transfer of DNA. This protein is also found to interact with BRCA1 and BRCA2, which may be important for the cellular response to DNA damage. BRCA2 is shown to regulate both the intracellular localization and DNA-binding ability of this protein. Loss of these controls following BRCA2 inactivation may be a key event leading to genomic instability and tumorigenesis. Two alternatively spliced transcript variants of this gene, which encode distinct proteins, have been reported. Transcript variants utilizing alternative polyA signals exist.

     

    应用推荐稀释比例
    {WB}{1:500-2000}
    {IHC-P}{1:100-500}
    {IHC-F}{1:100-500}
    {IF}{1:100-500}
    {Flow-Cyt}{2ug/Test}

     

    产品细节图片1
    P‌‌araformaldehyde-fixed, paraffin embedded (mouse intestine tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Rad51) Polyclonal Antibody, Unconjugated (bs-20297R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
    产品细节图片2
    Sample:Hela(human)cell Lysate at 40 ug
    Primary: Anti-Rad51(bs-20297R)at 1/300 dilution
    Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
    Predicted band size: 37kD
    Observed band size: 37kD
    产品细节图片3
    Sample:bone(mouse) Lysate at 40 ug
    Primary: Anti-Rad51(bs-20297R)at 1/300 dilution
    Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
    Predicted band size: 37kD
    Observed band size: 37kD
    产品细节图片4
    P‌‌araformaldehyde-fixed, paraffin embedded (Rat testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Rad51) Polyclonal Antibody, Unconjugated (bs-20297R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
    产品细节图片5
    P‌‌araformaldehyde-fixed, paraffin embedded (human colon cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Rad51) Polyclonal Antibody, Unconjugated (bs-20297R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
    产品细节图片6
    Blank control（black line）:Hela.
    Primary Antibody (green line): Rabbit Anti-Rad51 antibody (bs-20297R)
    Dilution:2ug/Test;
    Secondary Antibody（white blue line）: Goat anti-rabbit IgG-FITC
    Dilution: 0.5ug/Test.
    Isotype control（orange line）: Normal Rabbit IgG
    Protocol
    The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at -20℃, The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

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    [IF={{ 6.656 }}] {Leiyang Guo. et al. Morusinol extracted from Morus alba induces cell cycle arrest and apoptosis via inhibition of DNA damage response in melanoma by CHK1 degradation through the ubiquitin-proteasome pathway. PHYTOMEDICINE. 2023 Jun;114:154765} {WB} {Human}

    [IF={{ 5.168 }}] {Qi et al. Sensitization of tamoxifen-resistant breast cancer cells by Z-ligustilide through inhibiting autophagy and accumulating DNA damages. (2017) Oncotarget. 8:29300-29317} {ICC} {Human}

    [IF={{ 3.2 }}] {Talibova Gunel. et al. The DNA double-strand break repair proteins γH2AX, RAD51, BRCA1, RPA70, KU80, and XRCC4 exhibit follicle-specific expression differences in the postnatal mouse ovaries from early to older ages. J ASSIST REPROD GEN. 2024 Jul;:1-21} {IHC} {Mouse}

    [IF={{ 2.74 }}] {Niayale Robert. et al. Expression of Rad51 and the histo-morphological evaluation of testis of the sterile male cattle-yak. Theriogenology. 2021 Sep;172:239} {WB,IF,IHC} {Bovine}

    [IF={{ 2.531 }}] {Talibova, Gunel. et al. Increased double-strand breaks in aged mouse male germ cells may result from changed expression of the genes essential for homologous recombination or nonhomologous end joining repair. HISTOCHEM CELL BIOL. 2022 Oct;:1-21} {IHC} {Mouse}

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    Rad51 Rabbit pAb(bs-20297R)-50ul/100ul/200ul
    ￥1180 - 2800