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| 产品编号 | bs-12445P |
| 英文名称 | CAPG Antibody Blocking Peptide |
| 中文名称 | 肌动蛋白调节蛋白CAPG封闭多肽 |
| 英文别名 | Actin capping protein GCAP39; Actin regulatory protein CAP G; Actin regulatory protein CAP-G; AFCP; CAPG; CAPG_HUMAN; Capping protein (actin filament) gelsolin like; Capping protein gelsolin like; gCap39; Gelsolin like capping protein; Macrophage capping protein; Macrophage-capping protein; mbh1; MCP; Myc basic motif homolog 1. |
| 纯化方法 | HPLC |
| 研究领域 | Immunology > Innate Immunity > Macrophage / Inflamm. Signal Transduction > Cytoskeleton / ECM > Cytoskeleton > Microfilaments > Actin etc > Actin |
| 亚细胞定位 | Cytoplasm. Nucleus. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. |
| 组织特异性 | Macrophages and macrophage-like cells. |
| 翻译后修饰 | The N-terminus is blocked. |
| 相似性 | Belongs to the villin/gelsolin family. _x000D_ Contains 3 gelsolin-like repeats. |
| 功能 | Calcium-sensitive protein which reversibly blocks the barbed ends of actin filaments but does not sever preformed actin filaments. May play an important role in macrophage function. May play a role in regulating cytoplasmic and/or nuclear structures through potential interactions with actin. May bind DNA. |
| 保存条件 | Shipped at 4℃. Stored at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | Caldesmon, Filamin 1, Nebulin, Plastin, ADF, Gelsolin, CapG, Dematin and Cofilin are differentially expressed Actin-binding proteins. Both muscular (CDh) and non-muscular (CD1) forms of Caldesmon bind to Actin as well as to Calmodulin and Myosin. CDh is expressed predominantly on thin filaments in smooth muscle, whereas CD1 is widely expressed in non-muscle tissues and cells. CapG, also designated Actin-regulatory protein and macrophage-capping protein, is a macrophage-specific protein that reversibly blocks the barbed ends of Actin filaments, but does not sever preformed ones. The interactions of CapG with Actin may be important in the regulation of nuclear and cytoplasmic structures. CapG is a calcium-sensitive DNA-binding protein that plays a role in macrophage function. It is expressed in macrophages and macrophage-like cells and can localize both to the nucleus and the cytoplasm. |
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文献和实验Dual-Color ELISPOT Assay for the Simultaneous Detection of IL-2 and/or IFN- Secreting T Cells
, activate AEC buffer using H2 O2 (see Step 25). Anti-CD3 monoclonal antibody (mAb) (Fisher Scientific) Blocking buffer for ELISPOT assay CEF peptide pool (available from the NIH AIDS Research and Reagent Program and other commercial
0.3% hydrogen peroxide (prepare immediately before use) Blocking buffer (see recipe ) HCV‐specific primary antibody (Ab), e.g
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