SDS Antibody Blocking Peptide(

SDS

for 1.5 mm gel. 5. Block blot by soaking in blotting buffer for 1 hr with shaking. Alternatively,blocking can be done with as much as 10% milk and 0.5% Tween 20 to reduce background. 6. To 10 ml blocking solution, add primary antibody at proper dilution

Optimization Workflow for the Processing of High Quality Glass-Based Microarrays: Applications in DNA, Peptide, Antibody, and Carbohydrate Microarrayi

or from the literature are missing. In this article, processing parameters for DNA, peptide, antibody, and carbohydrate microarrays are outlined. The applicability of the model experiments is demonstrated and described in detail on the example of short oligonucleotides.

SDS PAGE and Western Blotting

at least three times for five minutes each in TBS-T 7. Optional: Reblock the filter after primary antibody for 10-30 minutes. 8. Apply the appropriate HRP-conjugated secondary antibody (diluted in blocking solution) to the filter for at least 1 hour