- 询价
- Sino Biological
- 北京
- 12206-H85H-SA
- 2026年01月26日
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- 详细信息
- 技术资料
- 保存条件：
-20℃ to -80℃
- 保质期：
12个月
- 英文名：
Recombinant Human CD98 Protein (Site-Specific APC Conjugation, His Tag)
- 库存：
99
- 供应商：
北京义翘神州科技股份有限公司
- 规格：
25.00 Tests/100.00 Tests
| 规格： | 25.00 Tests | 产品价格： | 询价 |
|---|---|---|---|
| 规格： | 100.00 Tests | 产品价格： | 询价 |
重组人CD98蛋白(APC定点偶联, His标签)（产品说明）
蛋白名称：Human CD98 Protein (Site-Specific APC Conjugation, His Tag)
蛋白构建：A DNA sequence encoding the human SLC3A2 isoform 1 (P08195-1) (Arg 206-Ala 630) was expressed with a N-terminal polyhistidine tag. The protein is site-specifically conjugated with APC (Excitation Max.= 651 nm, Emission Max.= 662 nm).
表达宿主：HEK293 Cells1.Flow cytometric analysis of anti-CD98 CAR expression. 5e5 of anti-CD98 CAR-293 cells were stained with 100 μL of 1:50 (2 μL stock solution in 100 μL FACS buffer) of APC-conjugated human CD98 Protein (Site-Specific APC Conjugation, His Tag, Cat. No. 12206-H85H-SA) and negative control protein. Non-transduced 293 cells were used as a control (left). APC signal was used to evaluate the binding activity (QC tested). 2.Binding activity of APC-conjugated CD98 protein to PBMC cells. PBMC cells were stained with FITC conjugated anti-CD3 antibody and APC-conjugated human CD98 Protein (Site-Specific APC Conjugation, His Tag, Cat. No. 12206-H85H-SA) and detected by flow cytometry. FITC signal was used to evaluate the content of CD3+ T cells in PBMCs. APC signal was used to evaluate the non-specific binding activity to PBMCs (QC tested).
蛋白内毒素：< 1.0 EU per μg protein as determined by the LAL method.
预测N端：Gly
蛋白分子量：The protein has a predicted molecular mass of 50.5 kDa.
蛋白NP号：P08195-1
蛋白氨基酸序列：Arg206-Ala630
蛋白名称：Human CD98 Protein (Site-Specific APC Conjugation, His Tag)
蛋白构建：A DNA sequence encoding the human SLC3A2 isoform 1 (P08195-1) (Arg 206-Ala 630) was expressed with a N-terminal polyhistidine tag. The protein is site-specifically conjugated with APC (Excitation Max.= 651 nm, Emission Max.= 662 nm).
表达宿主：HEK293 Cells1.Flow cytometric analysis of anti-CD98 CAR expression. 5e5 of anti-CD98 CAR-293 cells were stained with 100 μL of 1:50 (2 μL stock solution in 100 μL FACS buffer) of APC-conjugated human CD98 Protein (Site-Specific APC Conjugation, His Tag, Cat. No. 12206-H85H-SA) and negative control protein. Non-transduced 293 cells were used as a control (left). APC signal was used to evaluate the binding activity (QC tested). 2.Binding activity of APC-conjugated CD98 protein to PBMC cells. PBMC cells were stained with FITC conjugated anti-CD3 antibody and APC-conjugated human CD98 Protein (Site-Specific APC Conjugation, His Tag, Cat. No. 12206-H85H-SA) and detected by flow cytometry. FITC signal was used to evaluate the content of CD3+ T cells in PBMCs. APC signal was used to evaluate the non-specific binding activity to PBMCs (QC tested).
蛋白内毒素：< 1.0 EU per μg protein as determined by the LAL method.
预测N端：Gly
蛋白分子量：The protein has a predicted molecular mass of 50.5 kDa.
蛋白NP号：P08195-1
蛋白氨基酸序列：Arg206-Ala630
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重组人 CD98蛋白 (APC定点标记, His 标签)
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