- 询价
- Sino Biological
- 北京
- 11241-H86H-SD
- 2025年10月15日
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- 详细信息
- 技术资料
- 保存条件：
-20℃ to -80℃
- 保质期：
12个月
- 英文名：
Recombinant Human FOLR1 Protein (Site-Specific AF 488 Conjugation, His Tag)
- 库存：
99
- 供应商：
北京义翘神州科技股份有限公司
- 规格：
25.00 µg/100.00 µg
| 规格： | 25.00 µg | 产品价格： | 询价 |
|---|---|---|---|
| 规格： | 100.00 µg | 产品价格： | 询价 |
重组人FOLR1蛋白（AF 488定点偶联，His标签）（产品说明）
蛋白名称：Human FOLR1 Protein (Site-Specific AF 488 Conjugation, His Tag)
蛋白构建：A DNA sequence encoding the human FOLR1 (NP_057937.1) (Met 1-Met 233) without the pro peptide was expressed with a C-terminal polyhistidine tag. The protein is site-specifically conjugated with AF 488 (Excitation Max.= 495 nm, Emission Max.= 519 nm).
表达宿主：HEK293 Cells1.Flow cytometric analysis of anti-FOLR1 CAR expression. 5e5 of anti-FOLR1 CAR-293 cells were stained with 100 μL of 5 μg/mL of AF 488-conjugated human FOLR1 Protein (Site-Specific AF 488 Conjugation, His Tag, Cat. No. 11241-H86H-SD) and negative control protein. Non-transduced 293 cells were used as a control (left). AF 488 signal was used to evaluate the binding activity (QC tested). 2.Binding activity of AF 488-conjugated FOLR1 protein to PBMC cells. PBMC cells were stained with PE conjugated anti-CD3 antibody and AF 488-conjugated human FOLR1 Protein (Site-Specific AF 488 Conjugation, His Tag, Cat. No. 11241-H86H-SD) and detected by flow cytometry. PE signal was used to evaluate the content of CD3+ T cells in PBMCs. AF 488 signal was used to evaluate the non-specific binding activity to PBMCs (QC tested).
蛋白内毒素：< 1.0 EU per μg protein as determined by the LAL method.
预测N端：Arg 25
蛋白分子量：The protein has a predicted molecular mass of 27.9 kDa.
蛋白NP号：NP_057937.1
蛋白氨基酸序列：Met1-Met233
蛋白名称：Human FOLR1 Protein (Site-Specific AF 488 Conjugation, His Tag)
蛋白构建：A DNA sequence encoding the human FOLR1 (NP_057937.1) (Met 1-Met 233) without the pro peptide was expressed with a C-terminal polyhistidine tag. The protein is site-specifically conjugated with AF 488 (Excitation Max.= 495 nm, Emission Max.= 519 nm).
表达宿主：HEK293 Cells1.Flow cytometric analysis of anti-FOLR1 CAR expression. 5e5 of anti-FOLR1 CAR-293 cells were stained with 100 μL of 5 μg/mL of AF 488-conjugated human FOLR1 Protein (Site-Specific AF 488 Conjugation, His Tag, Cat. No. 11241-H86H-SD) and negative control protein. Non-transduced 293 cells were used as a control (left). AF 488 signal was used to evaluate the binding activity (QC tested). 2.Binding activity of AF 488-conjugated FOLR1 protein to PBMC cells. PBMC cells were stained with PE conjugated anti-CD3 antibody and AF 488-conjugated human FOLR1 Protein (Site-Specific AF 488 Conjugation, His Tag, Cat. No. 11241-H86H-SD) and detected by flow cytometry. PE signal was used to evaluate the content of CD3+ T cells in PBMCs. AF 488 signal was used to evaluate the non-specific binding activity to PBMCs (QC tested).
蛋白内毒素：< 1.0 EU per μg protein as determined by the LAL method.
预测N端：Arg 25
蛋白分子量：The protein has a predicted molecular mass of 27.9 kDa.
蛋白NP号：NP_057937.1
蛋白氨基酸序列：Met1-Met233
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重组人FOLR1蛋白（AF 488定点偶联，His标签）
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