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- Sino Biological
- 北京
- CT170-V10708H
- 2025年10月15日
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- 详细信息
- 技术资料
- 保存条件：
-20℃ to -80℃
- 保质期：
12个月
- 英文名：
Recombinant HSV-1 (strain 17) gH & gL Heterodimer Protein (Strep II & His Tag)
- 库存：
99
- 供应商：
北京义翘神州科技股份有限公司
- 规格：
100.00 µg
重组单疱病毒gH & gL Heterodimer蛋白 (Strep II & His 标签)（产品说明）
蛋白名称：HSV-1 (strain 17) gH & gL Heterodimer Protein (Strep II & His Tag) (MALS-verified)
蛋白构建：A DNA sequence encoding the HSV-1 gH (P06477) (Met1-Ala792) was fused with a Strep II at the C-terminus., constructed the plasmid 1; A DNA sequence encoding the HSV-1 gL (P10185) (Met1-Leu224) was fused with a polyhistidine tag at the C-terminus, constructed the plasmid 2. The two plasmids were co-expressed and the HSV-1 (strain 17) gH & gL Heterodimer was purified. The purified protein was biotinylated in vitro.
表达宿主：HEK293 Cells
蛋白纯度：≥ 90 % as determined by SDS-PAGE. ≥ 90 % as determined by SEC-MALS.
蛋白内毒素：< 1.0 EU per μg protein as determined by the LAL method.
预测N端：Gln 19 & Gly 20
蛋白分子量：The recombinant HSV-1 (strain 17) gH & gL Heterodimer consists of (782+215) amino acids and predicts a molecular mass of (84.69+24.41) kDa. It migrates as approximately 102.2,39.3 and 36.4 kDa band in SDS-PAGE under reducing conditions. The molecular weight of this protein is around 130.1 kDa verified by SEC-MALS.
蛋白NP号：P06477 & P10185
蛋白氨基酸序列：Met1-Ala792 & Met1-Leu224
蛋白名称：HSV-1 (strain 17) gH & gL Heterodimer Protein (Strep II & His Tag) (MALS-verified)
蛋白构建：A DNA sequence encoding the HSV-1 gH (P06477) (Met1-Ala792) was fused with a Strep II at the C-terminus., constructed the plasmid 1; A DNA sequence encoding the HSV-1 gL (P10185) (Met1-Leu224) was fused with a polyhistidine tag at the C-terminus, constructed the plasmid 2. The two plasmids were co-expressed and the HSV-1 (strain 17) gH & gL Heterodimer was purified. The purified protein was biotinylated in vitro.
表达宿主：HEK293 Cells
蛋白纯度：≥ 90 % as determined by SDS-PAGE. ≥ 90 % as determined by SEC-MALS.
蛋白内毒素：< 1.0 EU per μg protein as determined by the LAL method.
预测N端：Gln 19 & Gly 20
蛋白分子量：The recombinant HSV-1 (strain 17) gH & gL Heterodimer consists of (782+215) amino acids and predicts a molecular mass of (84.69+24.41) kDa. It migrates as approximately 102.2,39.3 and 36.4 kDa band in SDS-PAGE under reducing conditions. The molecular weight of this protein is around 130.1 kDa verified by SEC-MALS.
蛋白NP号：P06477 & P10185
蛋白氨基酸序列：Met1-Ala792 & Met1-Leu224
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重组单疱病毒gH & gL Heterodimer蛋白 (Strep II & His 标签)
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