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- bsm-30151H-Percp-Cy5.5
- 2025年10月15日
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产品信息以Bioss网站为准
- 规格：
25T/50T/100T
| 规格： | 25T | 产品价格： | ￥1080.0 |
|---|---|---|---|
| 规格： | 50T | 产品价格： | ￥1680.0 |
| 规格： | 100T | 产品价格： | ￥2680.0 |
| 产品编号 | bsm-30151H-Percp-Cy5.5 |
| 英文名称 | Mouse CD3e Hamster mAb, Percp-Cy5.5 conjugated |
| 中文名称 | Percp-Cy5.5标记小鼠CD3e单克隆抗体 |
| 英文别名 | CD3 epsilon; CD3e; CD3e antigen epsilon polypeptide(TiT3 complex); CD3E antigen epsilon polypeptide; CD3E antigen, epsilon subunit; CD3e molecule epsilon; CD3e molecule, epsilon(CD3 TCR complex); CD3e molecule, epsilon(CD3-TCR complex); CD3E_MOUSE; IMD18; T cell antigen receptor complex epsilon subunit of T3; T cell surface antigen T3/Leu 4 epsilon chain; T cell surface glycoprotein CD3 epsilon chain; T-cell surface antigen T3/Leu-4 epsilon chain; T-cell surface glycoprotein CD3 epsilon chain; T3E; TCRE. |
| 产品应用 | Flow-Cyt=10ul/Test Not yet tested in other applications. |
| 抗体来源 | Hamster |
| 亚型 | Armenian Hamster IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein G |
| 克隆类型 | Monoclonal |
| 理论分子量 | 20 kDa |
| 实际分子量 | 20kDa |
| 储存液 | 0.01M PBS (pH7.4), 0.5% BSA, 0.02% Proclin300. |
| 研究领域 | Immunology > Adaptive Immunity > Regulatory T Cells Immunology > Adaptive Immunity > T Cells > CD Signal Transduction > Antibodies > cd3 Stem Cells > Hematopoietic Progenitors > Hematopoietic Stem Cells > Human Lineage Negative |
| 亚基 | he TCR/CD3 complex of T-lymphocytes consists of either a TCR alpha/beta or TCR gamma/delta heterodimer coexpressed at the cell surface with the invariant subunits of CD3 labeled gamma, delta, epsilon, zeta, and eta. |
| 亚细胞定位 | Membrane; Single-pass type I membrane protein. |
| 相似性 | Contains 1 Ig-like (immunoglobulin-like) domain. Contains 1 ITAM domain. |
| 功能 | The CD3 complex mediates signal transduction. |
| 保存条件 | Store at 2-8℃. Protect from light. Avoid freezing. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | CD3e molecule, epsilon is also known as CD3E, is a T-cell surface single-pass type I membrane glycoprotein. CD3E contains 1 Ig-like (immunoglobulin-like) domain and 1 ITAM domain. CD3E, together with CD3-gamma, CD3-delta and CD3-zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T cell receptor-CD3 complex. This complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. The genes encoding the epsilon, gamma and delta polypeptides are located in the same cluster on chromosome 11. The epsilon polypeptide plays an essential role in T-cell development. CD3E plays an essential role in T-cell development, and defects in CD3E gene cause severe immunodeficiency. CD3E gene has also been linked to a susceptibility to type I diabetes in women. CD3E has been shown to interact with TOP2B, CD3EAP and NCK2. |
| 应用 | 推荐稀释比例 |
| {Flow-Cyt} | {10ul/Test} |
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文献和实验细胞的抗原表达模式也存在差异，研究中需结合组织特异性合理搭配抗体。 Mouse CD3，CD3e和CD3复合体 CD3与CD3e是流式细胞术中检测小鼠总T细胞群的首xuan标志物，适用于绝大多数实验品系。它们广泛表达于所有T细胞亚群表面（涵盖αβTCR和γδTCR），能够实现对T细胞的全面鉴定。需要特别注意的是，在某些基因修饰小鼠模型中（例如TCR敲除模型），由于基因层面的改变，CD3ε链的表达可能会受到限制或出现异常。在这种情况下，单独使用针对CD3e的抗体可能导致T细胞漏检。因此，建议改用识别
Direct Immunofluorescence Labeling
Recipes Fluorochrome-Conjugated Antibodies: Test antibody: Mouse monoclonal conjugated to FITC, PE, PerCP or PE-CY5 Tandem (tradenames: Cy-Chrome or Tri-Color). If you''re planning to label cells with 2 or more antibodies simultaneously
Fluorochrome Absorption and Emission Spectra
. Due to its photobleaching characteristics, PerCP conjugates are not recommended for use on stream-in-air flow cytometers. PerCP-Cy5.5 is a tandem conjugate system than combines PerCP with a cyanine dye (Cy5.5™) and has an absorption maximum
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