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- bsm-30050M-FITC
- 2025年10月15日
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产品信息以Bioss网站为准
- 规格：
25T/50T/100T
| 规格： | 25T | 产品价格： | ￥680.0 |
|---|---|---|---|
| 规格： | 50T | 产品价格： | ￥1080.0 |
| 规格： | 100T | 产品价格： | ￥1680.0 |
| 产品编号 | bsm-30050M-FITC |
| 英文名称 | human CD62P Mouse mAb, FITC conjugated |
| 中文名称 | FITC标记人CD62P单克隆抗体 |
| 英文别名 | P-selectin; CD_antigen: CD62P; CD62 antigen-like family member P; GMP 140; GMP-140; GMRP; Granule membrane protein 140; Granulocyte membrane protein; GRMP; LECAM 3; LECAM3; Leukocyte endothelial cell adhesion molecule 3; Leukocyte-endothelial cell adhesion molecule 3; P Selectin; PADGEM; Platelet activation dependent granule-external membrane protein; PSEL; sp-selectin; LYAM3_HUMAN. |
| 产品应用 | Flow-Cyt=10ul/Test Not yet tested in other applications. |
| 抗体来源 | Mouse |
| 亚型 | Mouse IgG1, k |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein G |
| 克隆类型 | Monoclonal |
| 理论分子量 | 88 kDa |
| 实际分子量 | 140kDa |
| 储存液 | 0.01M PBS (pH7.4), 0.5% BSA, 0.02% Proclin300. |
| 研究领域 | Cardiovascular > Angiogenesis > Endothelial Cell Markers Cardiovascular > Atherosclerosis > Vascular Inflammation > Leukocyte recruitment > Cell adhesion molecules Cardiovascular > Blood > Platelets Immunology > Adaptive Immunity > Regulatory T Cells Immunology > Cell Type Markers > CD > Platelets Stem Cells > Hematopoietic Progenitors > Myeloid > Thrombocytic Lineage |
| 亚基 | Interacts with SNX17. Interacts with PSGL1/SEPL and PODXL2 and mediates neutrophil adhesion and leukocyte rolling. This interaction requires the sialyl-Lewis X epitope of PSGL1 and PODXL2, and specific tyrosine sulfation on PSGL1. |
| 亚细胞定位 | Membrane; Single-pass type I membrane protein. |
| 组织特异性 | Stored in the alpha-granules of platelets and Weibel-Palade bodies of endothelial cells. Upon cell activation by agonists, P-selectin is transported rapidly to the cell surface. |
| 相似性 | Belongs to the selectin/LECAM family.
Contains 1 C-type lectin domain. Contains 1 EGF-like domain. |
| 功能 | Ca(2+)-dependent receptor for myeloid cells that binds to carbohydrates on neutrophils and monocytes. Mediates the interaction of activated endothelial cells or platelets with leukocytes. The ligand recognized is sialyl-Lewis X. Mediates rapid rolling of leukocyte rolling over vascular surfaces during the initial steps in inflammation through interaction with PSGL1. |
| 保存条件 | Store at 2-8℃. Protect from light. Avoid freezing. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | This gene encodes a 140 kDa protein that is stored in the alpha-granules of platelets and Weibel-Palade bodies of endothelial cells. This protein redistributes to the plasma membrane during platelet activation and degranulation and mediates the interaction of activated endothelial cells or platelets with leukocytes. The membrane protein is a calcium-dependent receptor that binds to sialylated forms of Lewis blood group carbohydrate antigens on neutrophils and monocytes. Alternative splice variants may occur but are not well documented. [provided by RefSeq, Jul 2008] |
| 应用 | 推荐稀释比例 |
| {Flow-Cyt} | {10ul/Test} |
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文献和实验Dual- and Triple-Color Fluorospot
antibodies (mAbs) to cytokines: Mouse mAb to human IFN-γ; mAb 1-D1K for coating and FITC-labeled mAb 7-B6-1 for detection. Mouse mAb to human IL-2; mAbs IL2-I/249 for coating and biotinylated mAb IL2-II for detection. If a third cytokine is included
Immunohistochemistry using Anti-Ganglioside Antibodies
Acetone Mouse MAb to ganglioside Mouse MAb for control experiment FITC-conjugated goat F(ab')2 fragment to mouse IgM (µ chain-specific) FITC-conjugated goat F(ab')2 fragment to mouse IgG
Flow Cytometry: Immunofluorescence Staining of Activated and Resting Human Platelets
for 30 minutes at room temperature.6. Wash two times in PBS and resuspend cell pellet in PBS. Cells may be stored at 4℃ for up to 8 hours.7. Stain activated platelets by direct immunofluorescence using CD62P-FITC, catalog number 555523 (31794×), at 20 μL
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