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- Bioss
- bs-18819R-HRP
- 2025年10月15日
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100ul
| 产品编号 | bs-18819R-HRP |
| 英文名称 | METTL21D Rabbit pAb, HRP conjugated |
| 中文名称 | HRP标记的甲基化样蛋白21D抗体 |
| 英文别名 | C14orf138; Methyltransferase like 21D; Methyltransferase-like protein 21D; Mettl21d; MT21D_HUMAN; Protein lysine methyltransferase METTL21D; Valosin containing protein lysine(K) methyltransferase; Valosin containing protein lysine methyltransferase; VCP KMT; VCP lysine methyltransferase; VCPKMT. |
| 产品应用 | ELISA=1:1000-10000, IHC-P=1:100-500, IHC-F=1:100-500 Not yet tested in other applications. |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human METTL21D |
| 亚型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Cell Biology > Other Antibodies > Other Antibodies |
| 亚基 | Interacts with ALKBH6. Interacts with ASPSCR1 and UBXN6; interaction with ASPSCR1, but not with UBXN6, enhances VCP methylation. |
| 亚细胞定位 | Cytoplasm |
| 相似性 | Belongs to the methyltransferase superfamily. METTL21 family. |
| 功能 | Protein-lysine N-methyltransferase that specifically trimethylates 'Lys-315' of VCP/p97; this modification may decrease VCP ATPase activity. |
| 保存条件 | Shipped at 4℃. Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| 应用 | 推荐稀释比例 |
| {ELISA} | {1:1000-10000} |
| {IHC-P} | {1:100-500} |
| {IHC-F} | {1:100-500} |
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文献和实验affinity chromatography and conjugated with horseradish peroxidase (HRP). Formulation Supplied as a 0.2 μm filtered solution in phosphate-buffered saline (PBS) (pH 7.0) with stabilizers. Storage Neat samples can be stored at 2° - 8° C
Western Immunoblotting of Proteins
. For a mouse monoclonal antibody, a goat-anti-mouse HRP conjugated antibody is recommended, for a rabbit polyclonal antibody, a goat-anti-rabbit HRP conjugated antibody is advisable. 7. Wash the nitrocellulose membrane five times for 3 to 5 minutes
Western Immunoblotting of Proteins
antibody, a goat-anti-mouse HRP conjugated antibody is recommended, for a rabbit polyclonal antibody, a goat-anti-rabbit HRP conjugated antibody is advisable. 7. Wash the nitrocellulose membrane five times for 3 to 5 minutes each with either water
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