Blank control:U-937.
Primary Antibody (green line): Rabbit Anti-CD33 antibody (bsm-30078M-APC)
Dilution: 1ug/Test;
Protocol
The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature.Acquisition of 20,000 events was performed.
- ￥2980
- Bioss
- bsm-30078M-APC
- 2025年10月15日
- 产品信息以Bioss网站为准
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产品信息以Bioss网站为准
- 规格：
100ul
| 产品编号 | bsm-30078M-APC |
| 英文名称 | human CD33 Mouse mAb, APC conjugated |
| 中文名称 | APC标记的小鼠抗人CD33单克隆抗体 |
| 产品应用 | Flow-Cyt=1ug/test Not yet tested in other applications. |
| 抗体来源 | Mouse |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein G |
| 克隆类型 | Monoclonal |
| 理论分子量 | 40/37 kDa |
| 储存液 | 0.01M PBS (pH7.4), 0.5% BSA, 0.02% Proclin300. |
| 研究领域 | Stem Cells |
| 亚基 | Subunit（亚单位） |
| 亚细胞定位 | Subcellular Location（亚细胞定位） |
| 组织特异性 | Tissue Specificity（组织特异性） |
| 翻译后修饰 | Post-translational modifications（蛋白质翻译后修饰） |
| 相似性 | Similarity（相似性） |
| 功能 | Function（功能、作用） |
| 保存条件 | Store at 2-8℃. Protect from light. Avoid freezing. |
| 背景资料 | 英文介绍 |
| 应用 | 推荐稀释比例 |
| {Flow-Cyt} | {1ug/test} |
Flow cytometry staining of normal human peripheral blood cells with CD33/APC(bsm-30078M-APC)(Red histogram) or Mouse IgG2a Isotype Control (APC Conjugate)(C03-12004)(Blue histogram) . Total cells were used for analysis.
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文献和实验相关实验
Fluorochrome Absorption and Emission Spectra
light at 488 nm for three-color flow cytometric analysis (Fig. 1). BD Biosciences Pharmingen also offers monoclonal antibodies and proteins conjugated to allophycocyanin (APC) and avidin conjugated to Texas Red™. Both of these dyes
Fluorochrome Absorption and Emission
for three-color flow cytometric analysis (Fig. 1). BD Biosciences Pharmingen also offers monoclonal antibodies and proteins conjugated to allophycocyanin (APC) and avidin conjugated to Texas Red™. Both of these dyes are useful in experiments
, A. and Su, Y. 2008. Mouse xenograft models vs GEM models for human cancer therapeutics. Dis. Models Mech. 1:78‐82. Ross, D.T. and Perou, C.M. 2001. A comparison of gene expression
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human CD33 Mouse mAb, APC conjugated(bsm-30078M-APC)-100ul
￥2980
