Blank control:LOVO.
Primary Antibody (green line): Rabbit Anti-CD133 antibody (bs-4770R-FITC)
Dilution: 2μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 0.1%PBST for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature.Cells stained with Primary Antibody for 30 min at room temperature. Acquisition of 20,000 events was performed.
- ￥2980
- Bioss
- bs-4770R-FITC
- 2025年10月15日
- 产品信息以Bioss网站为准
企业认证
相关产品推荐更多 >
ZDHHC11/ZNF399 Rabbit pAb(bs-18472R)-50ul/100ul/200ul
￥1180
EpCAM Rabbit pAb, Cy3 conjugated(bs-4889R-Cy3)-100ul
￥2980SNAI2 Rabbit pAb, PE-Cy5.5 conjugated(bs-1382R-PE-Cy5.5)-100ul
￥2980Beta galactosidase Rabbit pAb, BF555 conjugated(bs-4631R-BF555)-100ul
￥2980
Anti-human RHD / CD240d (LFB Anti-RhD Biosimilar)(BIO0758SM)-1mg/5mg/20mg
￥3200
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 文献和实验
- 技术资料
- 应用范围：
产品信息以Bioss网站为准
- 规格：
100ul
| 产品编号 | bs-4770R-FITC |
| 英文名称 | CD133 Rabbit pAb, FITC conjugated |
| 中文名称 | FITC标记的造血干细胞抗原CD133抗体 |
| 英文别名 | AC133; Antigen AC133; Hematopoietic stem cell antigen; hProminin; PROM1; Prominin I; Prominin 1; Prominin1; Prominin-1; Prominin like protein 1 precursor; Prominin mouse like 1; prominin1; PROML1; CD133; CORD12; MCDR2; MSTP061; PROML1; RP41; STGD4. |
| 产品应用 | Flow-Cyt=1μg/Test Not yet tested in other applications. |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human CD133 |
| 亚型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Neuroscience > Cell Type Marker > Neural Stem Cell marker Stem Cells > Hematopoietic Progenitors > Surface Molecules Stem Cells > Neural Stem Cells > Surface Molecules |
| 亚基 | Interacts with CDHR1 and with actin filaments. |
| 亚细胞定位 | Cell projection, cilium, photoreceptor outer segment. Isoform 1: Apical cell membrane; Multi-pass membrane protein. Cell projection, microvillus membrane; Multi-pass membrane protein. Note=Found in extracellular membrane particles in various body fluids such as cerebrospinal fluid, saliva, seminal fluid and urine. |
| 组织特异性 | Isoform 1 is selectively expressed on CD34 hematopoietic stem and progenitor cells in adult and fetal bone marrow, fetal liver, cord blood and adult peripheral blood. Isoform 1 is not detected on other blood cells. Isoform 1 is also expressed in a number of non-lymphoid tissues including retina, pancreas, placenta, kidney, liver, lung, brain and heart. Found in saliva within small membrane particles. Isoform 2 is predominantly expressed in fetal liver, skeletal muscle, kidney, and heart as well as adult pancreas, kidney, liver, lung, and placenta. Isoform 2 is highly expressed in fetal liver, low in bone marrow, and barely detectable in peripheral blood. Isoform 2 is expressed on hematopoietic stem cells and in epidermal basal cells (at protein level). Expressed in adult retina by rod and cone photoreceptor cells (at protein level). |
| 翻译后修饰 | Isoform 1 and isoform 2 are glycosylated. |
| 相似性 | Belongs to the prominin family. |
| 功能 | Binds cholesterol in cholesterol-containing plasma membrane microdomains. Proposed to play a role in apical plasma membrane organization of epithelial cells. During early retinal development acts as a key regulator of disk morphogenesis. Involved in regulation of MAPK and Akt signaling pathways. In neuroblastoma cells suppresses cell differentiation such as neurite outgrowth in a RET-dependent manner. |
| 保存条件 | Shipped at 4℃. Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | This gene encodes a pentaspan transmembrane glycoprotein. The protein localizes to membrane protrusions and is often expressed on adult stem cells, where it is thought to function in maintaining stem cell properties by suppressing differentiation. Mutations in this gene have been shown to result in retinitis pigmentosa and Stargardt disease. Expression of this gene is also associated with several types of cancer. This gene is expressed from at least five alternative promoters that are expressed in a tissue-dependent manner. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2009] |
| 应用 | 推荐稀释比例 |
| {Flow-Cyt} | {1μg/Test} |
风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验该产品被引用文献
[IF={{ 5.116 }}] {Wei Wang. et al. The effect of endothelial progenitor cell transplantation on neointimal hyperplasia and reendothelialisation after balloon catheter injury in rat carotid arteries. Stem Cell Res Ther. 2021 Dec;12(1):1-12} {FCM} {Rat}
[IF={{ 3.309 }}] {Yu S et al. Isolation and characterization of endothelial colony-forming cells from mononuclear cells of rat bone marrow.Exp Cell Res. 2018 Sep 1;370(1):116-126.} {FCM} {Rat}
[IF={{ 0 }}] {Nugrahenny D et al. Physalis minima Leaves Extract Induces Re-Endothelialization in Deoxycorticosterone Acetate-Salt-Induced Endothelial Dysfunction in Rats. RESEARCH JOURNAL OF LIFE SCIENCE DECEMBER. 2017 04(03).} {FCM} {Rat}
相关实验
KitCD2-PECD3-FITC/PE/APCCD4-FITC/PE/APCCD6-FITC/PE/APCCD8-FITC/PE/APCCD25-Biotin/PECD30-PECD62L-FITC/PE/APCAnti-CRTH-2- Biotin/PEAnti-TCRα/β-PE/APC细胞因子分泌细胞IFN-r Secretion Assay Cell Enrichment and Detection Kit(PE)IL-2 Secretion Assay Cell Enrichment
Fluorochrome Absorption and Emission Spectra
conjugates to monoclonal antibodies and other proteins. Four of these fluorochromes, fluorescein isothiocyanate (FITC), R-phycoerythrin (R-PE), BD Cy-Chrome™, and PerCP, can be used with single-laser flow cytometers equipped with an argon-ion laser emitting
Fluorochrome Absorption and Emission
to monoclonal antibodies and other proteins. Four of these fluorochromes, fluorescein isothiocyanate (FITC), R-phycoerythrin (R-PE), BD Cy-Chrome™, and PerCP, can be used with single-laser flow cytometers equipped with an argon-ion laser emitting light at 488 nm
技术资料暂无技术资料 索取技术资料
文献支持
CD133 Rabbit pAb, FITC conjugated(bs-4770R-FITC)-100ul
￥2980
