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- bsm-33131M-HRP
- 2025年10月15日
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100ul
| 产品编号 | bsm-33131M-HRP |
| 英文名称 | mCherry Mouse mAb, HRP conjugated |
| 中文名称 | HRP标记的单体樱桃红荧光蛋白单克隆抗体 |
| 英文别名 | mCherry; monomeric cherry red fluorescent protein. |
| 产品应用 | WB=1:1000-5000 Not yet tested in other applications. |
| 抗体来源 | Mouse |
| 免疫原 | Recombinant Protein mCherry |
| 亚型 | IgG1 |
| 纯化方法 | affinity purified by Protein G |
| 克隆类型 | Monoclonal |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Kits/ Lysates/ Other > Fluorescent ligands |
| 保存条件 | Shipped at 4℃. Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | mCherry is a fluorophore (a fluorescent molecule) used in biotechnology as a tracer to follow the flow of fluids, as a marker when tagged to molecules and cells components. mCherry is a monomeric fluorescent construct with peak absorption/emission at 587 nm and 610 nm, respectively. It is resistant to photobleaching and is stable. mCherry is sometimes preferred to other fluorophores due to its colour, as well as its photostability compared to other monomeric fluorophores. |
| 应用 | 推荐稀释比例 |
| {WB} | {1:1000-5000} |
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文献和实验我是用来做筛选IgG型单克隆抗体,用它做二抗,筛到的阳性克隆化和扩大培养后用SIGMA亚类试剂盒鉴定却是IgM,郁闷!!!这个试剂盒能与IGM交叉?是不是抗轻链?我这种方式不对吗?谁知道有较好的抗鼠IgG而与IgM不交叉的抗体?? 试剂盒说明书:Anti-mouse IgG-HRP Antibody FOR RESEARCH USE ONLY. NOT FOR USE IN HUMANS. R&D Systems, Inc. 1-800-343-7475 2/21/06
for 6 months without reducing binding activity. 2) This buffer can not be used for diluting HRP conjugated antibodies since sodium azide is an inhibitor of HRP. HRP-conjugated Primary Antibody Dilution Buffer: 1%BSA
. For alkaline phosphatase, use a 1:3000 dilution of Biorad goat anti-mouse or anti-rabbit alkaline phosphatase conjugated antibody in blotto. Incubate on rocker 1 hr at 25℃. 8. Wash 3X3min in TBS/Tween. 9. Alkaline phosphatase development: Add developing
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