Blank control:Molt4.
Primary Antibody (green line): Rabbit Anti-Phospho-GCN2 (Thr899) antibody (bs-3155R-FITC)
Dilution: 2μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at -20℃. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. Acquisition of 20,000 events was performed.
- ￥2980
- Bioss
- bs-3155R-FITC
- 2025年10月15日
- 产品信息以Bioss网站为准
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产品信息以Bioss网站为准
- 规格：
100ul
| 产品编号 | bs-3155R-FITC |
| 英文名称 | phospho-GCN2 (Thr899) Rabbit pAb, FITC conjugated |
| 中文名称 | FITC标记的磷酸化蛋白激酶GCN2蛋白抗体 |
| 英文别名 | GCN2(phospho T899); p-GCN2(phospho T899); EIF2AK4; Eukaryotic Translation Initiation Factor 2-alpha kinase 4; GCN2-like protein; KIAA1338; E2AK4_HUMAN; Eif2ak4; Eukaryotic Translation Initiation Factor 2 alpha kinase 4; GCN2; GCN2 eIF2alpha kinase; GCN2-like protein; MGCN2. |
| 产品应用 | Flow-Cyt=1ug/Test Not yet tested in other applications. |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated Synthesised phosphopeptide derived from human GCN2 around the phosphorylation site of Thr899 |
| 亚型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Epigenetics and Nuclear Signaling > DNA / RNA > Translation > Regulation Signal Transduction > Protein Phosphorylation > Ser / Thr Kinases > Other Kinases |
| 组织特异性 | Widely expressed. |
| 翻译后修饰 | Autophosphorylated on threonine residues. |
| 相似性 | Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. GCN2 subfamily. Contains 2 protein kinase domains. Contains 1 RWD domain. |
| 功能 | Can phosphorylate the alpha subunit of EIF2 and may mediate translational control. |
| 保存条件 | Shipped at 4℃. Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | GCN2 belongs to a family of kinases that phosphorylate the alpha subunit of eukaryotic translation initiation factor 2 to downregulate protein synthesis in response to varied cellular stresses. |
| 应用 | 推荐稀释比例 |
| {Flow-Cyt} | {1ug/Test} |
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phospho-GCN2 (Thr899) Rabbit pAb, FITC conjugated(bs-3155R-FITC)-100ul
￥2980
