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1mg/5mg
| 规格: | 1mg | 产品价格: | ¥3280.0 |
|---|---|---|---|
| 规格: | 5mg | 产品价格: | ¥12800.0 |
| 产品编号 | bs-0370P |
| 英文名称 | Goat IgM |
| 中文名称 | 羊IgM(亲和纯化) |
| 英文别名 | Goat Immunoglobulin M, native; Native Goat IgM |
| 中文别名 | (亲和纯化) 羊IgM; 正常羊免疫球蛋白M |
| 性状 | Lyophilized |
| 储存液 | 0.01M PBS (pH7.4). |
| 保存条件 | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | IgM normally constitutes about 10% of serum immunoglobulins. IgM antibody is prominent in early immune responses to most antigens and is largely confined to plasma due to it's large size. Monomeric IgM is expressed as a membrane bound antibody on the surface of B cells and as a pentamer when secreted by plasma cells. Due to it's high valency IgM is more efficient than other isotypes is binding antigens with repeating epitopes (virus particles and red blood cells) and is more efficient than IgG in activiating the complement pathway. The gene for the mu constant region contains four domains separated by short intervening sequences. |
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文献和实验deposit: NoPrice_code: AAvailability in cell line catalogues: ATCC CCL 61; IZSBS BS CL15; DSMZ ACC 110;Bibliographic references:J Exp Med 1958;108:945 Proc Nat Acad Sci USA 1968;60:1275CHO-K1 (hamster, Chinese, ovary)Morphology: epithelial-likeSpecies
后置-20℃保存 20×SSC 10×PBS (DEPC处理的)含50%甲酰胺的杂交液(HS)--- 1ml分装后置-20℃保存 10×阻断液(10×BS) Buffer I Buffer II Buffer III 探针(P) 抗Dig-Ap NBT/BCIP 二、操作流程: 1) 脱蜡至水: 二甲苯5min×2 → 90%乙醇5min → DEPC水洗涤5min×2 2) 杂交前处理: 1×PBS洗涤5min → 蛋白酶K(终浓度20ug/ml in PBS)37℃
in Renal Cancer (p 73-96) Rosamonde E. Banks, Peter J. Selby Chapter 6: Heat Shock Protein 27 in Cancer (p 97-109) Dr. Cecilia Sarto, Dr. Fulvio Magni, BS. Cristina Valsecchi, Prof. Dr. Paolo Mocarelli Chapter 7: Proteomic Approaches for Biomarker
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