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20ug/100ug/500ug
| 规格： | 20ug | 产品价格： | ￥680.0 |
|---|---|---|---|
| 规格： | 100ug | 产品价格： | ￥2280.0 |
| 规格： | 500ug | 产品价格： | ￥6880.0 |
| 产品编号 | bs-43761P |
| 英文名称 | Recombinant human TREM2 protein, C-His-Avi (HEK293) |
| 中文名称 | 重组人TREM2蛋白 |
| 英文别名 | Triggering receptor expressed on myeloid cells 2; TREM-2; TREM2a; TREM2b; TREM2c; Trggering receptor expressed on myeloid cells 2a; TREM2_HUMAN; |
| 交叉反应 | (Human) |
| 性状 | Liquid |
| 纯化方法 | AC |
| 理论分子量 | 21.1 kDa |
| 浓度 | >1mg/ml |
| 储存液 | PBS (pH=7.4) |
| 研究领域 | Immunology > Innate Immunity > Macrophage / Inflamm. |
| 亚基 | Interacts with TYROBP/DAP12. |
| 亚细胞定位 | Isoform 1: Cell membrane; Single-pass type I membrane protein (Potential). Isoform 2: Secreted (Potential). Isoform 3: Secreted (Potential). |
| 组织特异性 | Expressed on macrophages and dendritic cells but not on granulocytes or monocytes. In the CNS strongest expression seen in the basal ganglia, corpus callosum, medulla oblongata and spinal cord. |
| 相似性 | Contains 1 Ig-like V-type (immunoglobulin-like) domain. |
| 功能 | May have a role in chronic inflammations and may stimulate production of constitutive rather than inflammatory chemokines and cytokines. Forms a receptor signaling complex with TYROBP and triggers activation of the immune responses in macrophages and dendritic cells. |
| 保存条件 | Stored at -70℃ or -20℃. Avoid repeated freeze/thaw cycles |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | This gene encodes a membrane protein that forms a receptor signaling complex with the TYRO protein tyrosine kinase binding protein. The encoded protein functions in immune response and may be involved in chronic inflammation by triggering the production of constitutive inflammatory cytokines. Defects in this gene are a cause of polycystic lipomembranous osteodysplasia with sclerosing leukoencephalopathy (PLOSL). Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Nov 2012] |
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文献和实验CO-IMMUNOPRECIPITATION ANALYSIS OF PROTEIN-PROTEIN INTERACTIONS
for this protocol. Transfect 2-T150s of HEK293 cells with the appropriate expression vectors (protein A and protein B) as per standard protocol. Alternatively, you can use an endogenous cell line that expressed both proteins if you have specific antibodies
Protein‐Protein Interactions Identified by Pull‐Down Experiments and Mass Spectrometry
.M., Speicher, D.W., and Wingfield, P.T. (eds.). 2003. Current Protocols in Protein Science. John Wiley & Sons, New York. Fernandez‐Patron, C., Hardy, E., Sosa, A., Seoane
Figure 5.23.3 Monitoring agonist‐promoted β‐arrestin2‐Rluc recruitment to β2AR‐EYFP and V2R‐EYFP by BRET1 . (A ) Real time BRET1 kinetics: HEK293T cells were cotransfected with pβ‐arrestin2‐hRluc and pβ2AR‐EYFP or pV
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