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- Bioss
- bs-0844P
- 2025年10月16日
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￥880
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500ug
| 产品编号 | bs-0844P |
| 英文名称 | GFP Antibody Blocking Peptide |
| 中文名称 | 绿色荧光蛋白封闭多肽 |
| 英文别名 | GFP; Green Fluorescent Protein. |
| 中文别名 | 绿色荧光蛋白 GFP; 绿色荧光蛋白 (GFP); |
| 性状 | Lyophilized |
| 纯化方法 | HPLC |
| 研究领域 | Tags & Cell Markers > Fusion / Marker Proteins > GFP |
| 亚基 | Monomer. |
| 组织特异性 | Photocytes. |
| 翻译后修饰 | Contains a chromophore consisting of modified amino acid residues. The chromophore is formed by autocatalytic backbone condensation between Ser-65 and Gly-67, and oxidation of Tyr-66 to didehydrotyrosine. Maturation of the chromophore requires nothing other than molecular oxygen. |
| 相似性 | Belongs to the GFP family. |
| 功能 | Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca(2+)-activated photoprotein aequorin |
| 保存条件 | Shipped at 4℃. Stored at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 背景资料 | Enhanced Blue Fluorescent Protein (EBFP) emits a strong blue fluorescence. EBFP can be used as protein "tags" to study the sub-cellular localization of proteins and/or their translocation upon stimulation or as markers for transfection in transient and stable expression systems. |
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文献和实验Anti-GFP/BrdU In Situ Immunostaining
Materials 24 well dishes Millicell 0.4 µm inserts (Millipore PICM01250) - coated with fibronectin Culture medium PBS Fixative: 4% paraformaldehyde in PBS (pH 7.6) Rabbit anti-GFP (Molecular Probes, A-11122) FITC-goat anti-rabbit
or from the literature are missing. In this article, processing parameters for DNA, peptide, antibody, and carbohydrate microarrays are outlined. The applicability of the model experiments is demonstrated and described in detail on the example of short oligonucleotides.
Immunocytochemistry Protocol Using Anti-GFP Antibodies(使用抗GFP抗体的免疫细胞化学实验方案)
. 7. Wash the cells twice in PBS for 1 minute each with gentle agitation. 8. Prepare a 1:400 dilution of anti-GFP chicken antibody in PBS to obtain a final antibody concentration of 5.0 µg/mL. 9. Remove the PBS
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