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| 产品编号 | bs-14539P |
| 英文名称 | EIF2D Antibody Blocking Peptide |
| 中文名称 | EIF2D蛋白封闭多肽 |
| 英文别名 | Eukaryotic translation initiation factor 2D; HCA56; Hepatocellular carcinoma associated antigen 56; Hepatocellular carcinoma-associated antigen 56; LGTN; LIGA_HUMAN; Ligatin; OTTHUMP00000034537; OTTHUMP00000034538. |
| 纯化方法 | HPLC |
| 研究领域 | Signal Transduction > Protein Trafficking > Organelle Proteins |
| 亚细胞定位 | Membrane. |
| 相似性 | Belongs to the ligatin family. _x000D_ Contains 1 PUA domain. _x000D_ Contains 1 SUI1 domain. |
| 功能 | Trafficking receptor for phosphoglycoproteins. Localizes phosphoglycoproteins within endosomes and at the cell periphery where they participate in specific metabolic processes as well as intercellular adhesion. |
| 保存条件 | Shipped at 4℃. Stored at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | HCA56 is a 584 amino acid peripheral membrane protein belonging to the ligatin family. Expressed during embryonic development and in early differentiated states, ligatin is a trafficking receptor for phosphoglycoproteins. Ligatin localizes to phosphoglycoproteins within endosomes and at the cell periphery where it participates in specific metabolic processes as well as intercellular adhesion. Involved in RNA binding and translation initiation through its single PUA domain and SUI1 domain, ligatin is down-regulated with long-lasting effects by the activation of Ca2+ dependent N-methyl-D-aspartate (NMDA) subclass of excitatory amino acid (EAA) receptors. Ligatin is considered a marker protein for membrane-vesicle transport systems. Ligatin exists as two alternatively splice variants and is encoded by a gene located on human chromosome 1. |
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文献和实验Dual-Color ELISPOT Assay for the Simultaneous Detection of IL-2 and/or IFN- Secreting T Cells
, activate AEC buffer using H2 O2 (see Step 25). Anti-CD3 monoclonal antibody (mAb) (Fisher Scientific) Blocking buffer for ELISPOT assay CEF peptide pool (available from the NIH AIDS Research and Reagent Program and other commercial
0.3% hydrogen peroxide (prepare immediately before use) Blocking buffer (see recipe ) HCV‐specific primary antibody (Ab), e.g
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