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| 规格： | 10ug | 产品价格： | ￥2080.0 |
|---|---|---|---|
| 规格： | 50ug | 产品价格： | ￥6800.0 |
| 规格： | 500ug | 产品价格： | ￥28000.0 |
| 产品编号 | bs-48005P |
| 英文名称 | Recombinant human IL-10 protein (Active, HEK293) |
| 中文名称 | 重组人白介素10 (活性的, Tag free) |
| 英文别名 | Interleukin-10 precursor; Cytokine synthesis inhibitory factor; CSIF; Cytokine Synthesis Inhibitory Factor; IL 10; IL10A; Interleukin 10; Interleukin10; MGC126450; MGC126451; TGIF; IL10_HUMAN. ;Recombinant human IL-10 protein (Active, HEK293) |
| 中文别名 | 人白介素10 重组蛋白; 人白细胞介素10 ; 重组人白介素10; 重组人IL-10; |
| 性状 | Lyophilized |
| 纯化方法 | AC |
| 理论分子量 | 17 |
| 实际分子量 | 21 kDa |
| 浓度 | ＞0.5 mg/ml |
| 储存液 | Lyophilized from 0.22um filtered solution in PBS (pH7.4) with 5mM DTT. Normally 5% trehalose is added as protectant before Lyophilization. |
| 研究领域 | Cancer > Tumor immunology > Cytokines > Interleukins Immunology > Adaptive Immunity > Regulatory T Cells Immunology > Innate Immunity > Cytokines > Interleukins |
| 亚基 | Homodimer. |
| 亚细胞定位 | Secreted. |
| 组织特异性 | Produced by a variety of cell lines, including T-cells, macrophages, mast cells and other cell types. |
| 相似性 | Belongs to the IL-10 family. |
| 功能 | IL-10 protein is a cytokine produced primarily by monocytes and to a lesser extent by lymphocytes. This cytokine has pleiotropic effects in immunoregulation and inflammation. It down-regulates the expression of Th1 cytokines, MHC class II Ags, and costimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. This cytokine can block NF-kappa B activity, and is involved in the regulation of the JAK-STAT signaling pathway. Knockout studies in mice suggested the function of this cytokine as an essential immunoregulator in the intestinal tract. |
| 保存条件 | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. In general: 12 months from date of receipt, -20 to -80℃ as supplied. 1 month, 2 to 8℃ under sterile conditions after reconstitution. 3 months, -20 to -80℃ under sterile conditions after reconstitution. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | The protein encoded by this gene is a cytokine produced primarily by monocytes and to a lesser extent by lymphocytes. This cytokine has pleiotropic effects in immunoregulation and inflammation. It down-regulates the expression of Th1 cytokines, MHC class II Ags, and costimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. This cytokine can block NF-kappa B activity, and is involved in the regulation of the JAK-STAT signaling pathway. Knockout studies in mice suggested the function of this cytokine as an essential immunoregulator in the intestinal tract. Mutations in this gene are associated with an increased susceptibility to HIV-1 infection and rheumatoid arthritis. [provided by RefSeq, May 2020] |
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文献和实验[资源] 申请上传 Current Protocols In Protein Science （60M） 见公共邮箱
5.7 Overview of Protein Expression in Pichia pastoris Unit 5.8 Culture of Yeast for the Production of Heterologous Proteins Unit 5.9 Overview of Protein Expression by Mammalian Cells Unit 5.10 Production of Recombinant Proteins in Mammalian Cells Unit 5.11
using monoclonal antibodies directed against a peptidic tag engineered at the C-terminal extremity of the recombinant antibody. In certain experimental conditions, such peptidic tags may undergo proteolytic cleavage, thereby lowering the sensitivity
detected using monoclonal antibodies directed against a peptidic tag engineered at the C-terminal extremity of the recombinant antibody. In certain experimental conditions, such peptidic tags may undergo proteolytic cleavage, thereby lowering
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