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500ug
| 产品编号 | bs-3649P |
| 英文名称 | KLF8 Antibody Blocking Peptide |
| 中文名称 | KLF样转录因子8封闭多肽 |
| 英文别名 | Basic krueppel-like factor 3; BKLF3; DXS741; Kruppel like factor 8; Zinc finger protein 741; ZNF741. |
| 纯化方法 | HPLC |
| 研究领域 | Epigenetics and Nuclear Signaling > Transcription > Domain Families > Krueppel like factor Epigenetics and Nuclear Signaling > Transcription > Domain Families > Zinc Finger Epigenetics and Nuclear Signaling > Transcription > Krueppel like factor |
| 亚基 | Interacts with corepressor CtBP2. Interacts with PIAS1, PIAS2, AND PIAS4; the interaction with each ligase sumoylates KLF8. |
| 亚细胞定位 | Nucleus. |
| 组织特异性 | Ubiquitous. |
| 翻译后修饰 | Sumoylation at Lys-67 represses transcriptional activity and reduces cell cycle progression into the G(1) phase. Has no effect on subcellular location. |
| 相似性 | Belongs to the Sp1 C2H2-type zinc-finger protein family. Contains 3 C2H2-type zinc fingers._x000D_ |
| 功能 | Transcriptional repressor and activator. Binds to CACCC-boxes promoter elements. Also binds the GT-box of cyclin D1 promoter and mediates cell cycle progression at G(1) phase as a downstream target of focal adhesion kinase (FAK). |
| 保存条件 | Shipped at 4℃. Stored at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | KLF8 is a transcriptional repressor and activator. It also binds the GT-box of cyclin D1 promoter and mediates cell cycle progression at G phase as a downstream target of focal adhesion kinase (FAK). Ubiquitously expressed. Kruppel-like factor proteins all contain a characteristic C-terminal DNA binding domain containing 3 Kruppel-like zinc fingers, and they all recognize CACCC and GC boxes present in promoters and enhancers. |
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文献和实验Dual-Color ELISPOT Assay for the Simultaneous Detection of IL-2 and/or IFN- Secreting T Cells
, activate AEC buffer using H2 O2 (see Step 25). Anti-CD3 monoclonal antibody (mAb) (Fisher Scientific) Blocking buffer for ELISPOT assay CEF peptide pool (available from the NIH AIDS Research and Reagent Program and other commercial
0.3% hydrogen peroxide (prepare immediately before use) Blocking buffer (see recipe ) HCV‐specific primary antibody (Ab), e.g
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