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- 2026年10月10日
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| 产品编号 | bs-13247P |
| 英文名称 | GNA13 Antibody Blocking Peptide |
| 中文名称 | G蛋白结合蛋白α13/Gα13封闭多肽 |
| 英文别名 | G alpha 13; GNA13; Guanine nucleotide binding protein alpha 13 subunit; GNA13_HUMAN. |
| 纯化方法 | HPLC |
| 研究领域 | Signal Transduction > Signaling Pathway > G Protein Signaling > Heterotrimeric G Proteins > G Proteins |
| 亚基 | G proteins are composed of 3 units; alpha, beta and gamma. The alpha chain contains the guanine nucleotide binding site. Interacts with UBXD5. Interacts with HAX1. |
| 亚细胞定位 | Cell Membrane; lipid-anchor. |
| 翻译后修饰 | Palmitoylation is critical for proper membrane localization and signaling. Phosphorylation on Thr-203 by PKA destabilizes the heterotrimer of alpha, beta and gamma, and inhibits Rho activation._x000D_ |
| 相似性 | Belongs to the G-alpha family. G(12) subfamily. |
| 功能 | Guanine nucleotide-binding proteins (G proteins) are modulators or transducers in various transmembrane signaling systems. They are composed of 3 subunits; alpha, beta and gamma. The alpha chain contains the guanine nucleotide binding site. |
| 保存条件 | Shipped at 4℃. Stored at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | Heterotrimeric G proteins function to relay information from cell surface receptors to intracellular effectors. Each of a very broad range of receptors specifically detects an extracellular stimulus (a photon, pheromone, odorant, hormone or neurotransmitter) while the effectors (i.e., adenyl cyclase), which act to generate one or more intracellular messengers, are less numerous. In mammals, G protein Alpha, Beta and Gamma polypeptides are encoded by at least 16, 4 and 7 genes, respectively. Most interest in G proteins has been focused on their Alpha subunits, since these proteins bind and hydrolyze GTP and most obviously regulate the activity of the best studied effectors. Four distinct classes of G Alpha subunits have been identified; these include G Alpha s, G Alpha i, G Alpha q and G Alpha 12/13. The two members of the fourth class of G Alpha subunit proteins, G Alpha 12 and G Alpha 13, are insensitive to ADP-ribosylation by pertussis toxin, share 67% identity with each other and less than 45% identity with other G Alpha subunits and are widely expressed in a broad range of tissues. |
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文献和实验Dual-Color ELISPOT Assay for the Simultaneous Detection of IL-2 and/or IFN- Secreting T Cells
, activate AEC buffer using H2 O2 (see Step 25). Anti-CD3 monoclonal antibody (mAb) (Fisher Scientific) Blocking buffer for ELISPOT assay CEF peptide pool (available from the NIH AIDS Research and Reagent Program and other commercial
0.3% hydrogen peroxide (prepare immediately before use) Blocking buffer (see recipe ) HCV‐specific primary antibody (Ab), e.g
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