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- IHC0620H
- 2025年10月15日
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50T
| 产品编号 | IHC0620H |
| 英文名称 | Human Topoisomerase I Ready-To-Use IHC Kit |
| 中文名称 | 人拓普西异构酶Ⅰ即用型免疫组化试剂盒 |
| 英文别名 | AI467334; D130064I21Rik; Top-1; DNA topoisomerase 1; DNA topoisomerase I; NUP98 fusion gene; TOP 1; TOP I; TOP1; TOP1_HUMAN; TOPI; Topoisomerase 1; Topoisomerase1; TopoisomeraseI; Type I DNA topoisomerase |
| 产品应用 | IHC-P Not yet tested in other applications. |
| 交叉反应 | Human |
| 亚基 | Monomer. Interacts with ERCC6 (PubMed:26030138). Interacts with TPRN; TPRN interacts with a number of DNA damage response proteins, is recruited to sites of DNA damage and may play a role in DNA damage repair (PubMed:23213405). (Microbial infection) Interacts with SV40 Large T antigen; this interactions allows viral DNA replication. |
| 亚细胞定位 | Nucleus |
| 组织特异性 | Endothelial cells. |
| 翻译后修饰 | Sumoylated. Lys-117 is the main site of sumoylation. Sumoylation plays a role in partitioning TOP1 between nucleoli and nucleoplasm. Levels are dramatically increased on camptothecin (CPT) treatment. Phosphorylation at Ser-506 by CK2 increases binding to supercoiled DNA and sensitivity to camptothecin. |
| 相似性 | Belongs to the type IB topoisomerase family. |
| 功能 | Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(3'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 5'-OH DNA strand. The free DNA strand then rotates around the intact phosphodiester bond on the opposing strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 5'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone (By similarity). Regulates the alternative splicing of tissue factor (F3) pre-mRNA in endothelial cells. Involved in the circadian transcription of the core circadian clock component BMAL1 by altering the chromatin structure around the ROR response elements (ROREs) on the BMAL1 promoter |
| 保存条件 | Please store components at the temperatures indicated on the individual tube labels. The kit is stable for 6 months from the date of receipt. |
| 背景资料 | This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This enzyme catalyzes the transient breaking and rejoining of a single strand of DNA which allows the strands to pass through one another, thus altering the topology of DNA. This gene is localized to chromosome 20 and has pseudogenes which reside on chromosomes 1 and 22. |
| 应用 | 推荐稀释比例 |
Immunohistochemical analysis of paraffin embedded Human colon cancer tissue slide using IHC0620H (Human Topoisomerase I Kit).
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Human Topoisomerase I Ready-To-Use IHC Kit(IHC0620H)-50T
￥3200
