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- D55703
- 2025年10月17日
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1mg/2mg
| 规格： | 1mg | 产品价格： | ￥443.0 |
|---|---|---|---|
| 规格： | 2mg | 产品价格： | ￥624.0 |
| 产品编号 | D55703 |
| 英文名称 | TRAM 39 |
| 英文别名 | 2-(2-chlorophenyl)-2,2-diphenylacetonitrile; 2-Chloro-alpha,alpha-diphenylbenzeneacetonitrile; TRAM-39; ZINC27105797 |
| 理论分子量 | 303.79 |
| 保存条件 | Powder: -20℃ for 3 years | In solvent: -80℃ for 1 year |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | TRAM 39 is a selective blocker of intermediate-conductance Ca2+-activated K+channels. |
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文献和实验: 35.5ul pure water 5ul 10 x PCR buffer (Boehringer Mannheim) 5ul 500uM dNTPs (some enzymes require a higher concentration of dNTPs (2mM), for example Pfu polymerase (though I'd recommend against the use
concentration of dNTPs (2mM), for example Pfu polymerase (though I'd recommend against the use of this enzyme) 1ul Adapter primer (25uM) 1ul Gene specific primer (25uM) 2ul cDNA template (various dilutions) 6. Mix and overlay with mineral oil.
Construction of homemade 'T-vectors&#;
Construction of homemade 'T-vectors' This method is after Marchuk, D., et al., 1991, Nucl. Acids Res. 19(5), pp 1154. You will need: 10 x Taq buffer (Promega) Taq Polymerase (Promega) Phenol/chloroform mix 100mM dTTP TE
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