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10 μg/50 μg/500 μg
| 规格: | 10 μg | 产品价格: | ¥1480.0 |
|---|---|---|---|
| 规格: | 50 μg | 产品价格: | ¥4880.0 |
| 规格: | 500 μg | 产品价格: | ¥20800.0 |
| 产品编号 | bs-48045P |
| 英文名称 | Recombinant mouse RSPO1 protein,C-His (Active,CHO) |
| 中文名称 | 重组小鼠RSPO1蛋白 |
| 英文别名 | R-spondin-1; Roof plate-specific spondin-1; RSPO1 |
| 性状 | Liquid |
| 纯化方法 | AC |
| 实际分子量 | 44 kDa |
| 储存液 | PBS (pH=7.4) |
| 保存条件 | Stored at -70°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -70°C for extended storage. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | RSPO1 activates the canonical Wnt signaling pathway by binding to the LGR4-6 receptor, triggering an increase in target gene expression. It inhibits ZNRF3, regulates the Wnt signaling pathway, and negatively regulates the TGF-β pathway. |
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文献和实验Harvesting GPI-Anchored Proteins From CHO Cells
expression. This approach has obtained product concentrations of up to 500 μg/mL at purities of 35% total protein (5 ,7 ).
of galactose induction steps and increasing the amount of Gal4p transcription factor both improved expression. Lowering the temperature from 28 to 18�C during expression enhanced the recovery of detergent-extractible active protein. In the “light membrane
_ChIP_method.html Chromatin IP Method, ChIP buffers, Notes on ChIP Method, Quantative PCR using 32P dCTP. Hahn Lab. ChIP Assay Protocol PDF - /attach/2008/2008-02-01/2B/2B6C4EA25F257424710A50E83CB2F380.pdf Formaldehyde cross-linking
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