- ￥2800
- Proteintech
- Biotin-67762
- 2025年10月13日
- IHC
- Mouse
- human
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Recombinant human IGLC1 protein
- 亚型：
IgG2a
- 保存条件：
Store at -20°C. Avoid exposure to light. Stable for one year after shipment.
- 克隆性：
Monoclonal
- 标记物：
Biotin
- 适应物种：
human
- 保质期：
详见产品包装
- 库存：
充足
- 宿主：
Mouse
- 应用范围：
IHC
- 靶点：
Human Ig lambda chain
- 抗体英文名：
Biotin-conjugated Human Ig lambda chain Monoclonal antibody
- 抗体名：
Biotin-conjugated Human Ig lambda chain Monoclonal antibody
- 规格：
100ul
经过测试的应用
| Positive IHC detected in | human tonsillitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
Biotin-67762 targets Human Ig lambda chain in IHC applications and shows reactivity with human samples.
| 经测试应用 | IHC |
| 经测试反应性 | human |
| 免疫原 |
CatNo: Ag14783 Product name: Recombinant human IGLC1 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-234 aa of BC007782 Sequence: MAWTVLLLGLLSHCTGSGTSYVLTQPASVSVAPGQTARITCGGSNLGSKSVNWYQLRPGQAPILVVYENKERPAGIPERLSALTSEETATLTISSVVAGDEADYFCQVWDTTSQQYVFGTGTQVTVLGQPKANPTVTLFPPSSEELQANKATLVCLISDFYPGAVTVAWKADGSPVKAGVETTKPSKQSNNKYAASSYLSLTPEQWKSHRSYSCQVTHEGSTVEKTVAPTECS |
| 宿主/亚型 | Mouse / IgG2a |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | immunoglobulin lambda constant 1 (Mcg marker) |
| 别名 | Human IgG lambda chain, IgG Lambda Light Chain, IGLC1, 1D12B5, IGLC |
| 计算分子量 | 233 aa, 25 kDa |
| 观测分子量 | ~25 kDa |
| GenBank蛋白编号 | BC007782 |
| 基因名称 | IgG Lambda Light Chain |
| Gene ID (NCBI) | 3537 |
| RRID | AB_2918953 |
| 偶联类型 | Biotin |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
This antibody detects Lambda chain of human immunoglobulin.
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文献和实验Dual- and Triple-Color Fluorospot
different Ig isotypes (6) and later for analysis of cytokines (7). Although successfully used in several studies (8, 9), the dual ELISPOT technique can be technically challenging to perform, suffers from several inherent analytical difficulties
them dry and then apply 3% human or pig serum as a blocking agent (health hazard!). 3- incubate with the blocking for 10 min. If one of your antibody is biotin-conjugated, you need at this point to do endogenous biotin blocking. 4- blot the slides
步骤如下： a. 以适当浓度的纯化抗鼠Ig抗体包被酶标板，每孔加100ul，37℃ 2小时或4℃过夜。 b. 洗涤、拍干后加待测的McAb样品，37℃ 1-2小时。 c. 洗涤后加适量的抗原，37℃ 1-2小时。 d. 洗涤后加入酶标多克隆抗体，37℃ 1-2小时。洗涤后加底物显色，判定结果。 F、ABC-ELISA试验 ABC-ELISA是在常规ELISA原理的基础上，增加了生物素（Biotin）与亲和素（Avidin）间
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