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100ul
| 产品编号 | bs-0494R-FITC |
| 英文名称 | ETFA Rabbit pAb, FITC conjugated |
| 中文名称 | FITC标记的电子转移黄素蛋白α抗体 |
| 英文别名 | ETF-alpha; Electron transfer flavoprotein subunit alpha; electron-transfer-flavoprotein, alpha polypeptide; mitochondrial; alpha ETF; alpha-ETF; Electron transfer flavoprotein alpha polypeptide; Electron transfer flavoprotein alpha subunit; Electron transfer flavoprotein subunit alpha; Electron transfer flavoprotein subunit alpha mitochondrial; Electron transfer flavoprotein subunit alpha, mitochondrial; Electron transferring flavoprotein alpha polypeptide; EMA; ETFA; ETFA_HUMAN; GA2. |
| 产品应用 | IF=1:100-500 Not yet tested in other applications. |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human ETFA |
| 亚型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Cancer > Cancer Metabolism > Metabolic signaling pathway > Integration of energy metabolism Metabolism > Pathways and Processes > Metabolic signaling pathways > Energy transfer pathways > Integration of energy Metabolism > Pathways and Processes > Mitochondrial Metabolism > Mitochondrial markers Metabolism > Types of disease > Cancer Signal Transduction > Metabolism > Mitochondrial |
| 亚基 | Heterodimer of an alpha and a beta subunit. |
| 亚细胞定位 | Mitochondrion matrix. |
| 翻译后修饰 | The N-terminus is blocked |
| 相似性 | Belongs to the ETF alpha-subunit/FixB family. |
| 功能 | The electron transfer flavoprotein serves as a specific electron acceptor for several dehydrogenases, including five acyl-CoA dehydrogenases, glutaryl-CoA and sarcosine dehydrogenase. It transfers the electrons to the main mitochondrial respiratory chain via ETF-ubiquinone oxidoreductase (ETF dehydrogenase). |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 背景资料 | ETFA participates in catalyzing the initial step of the mitochondrial fatty acid beta-oxidation. It shuttles electrons between primary flavoprotein dehydrogenases and the membrane-bound electron transfer flavoprotein ubiquinone oxidoreductase. Defects in electron-transfer-flavoprotein have been implicated in type II glutaricaciduria in which multiple acyl-CoA dehydrogenase deficiencies result in large excretion of glutaric, lactic, ethylmalonic, butyric, isobutyric, 2-methyl-butyric, and isovaleric acids. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]. |
| 应用 | 推荐稀释比例 |
| {IF} | {1:100-500} |
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文献和实验Fluorochrome Absorption and Emission Spectra
conjugates to monoclonal antibodies and other proteins. Four of these fluorochromes, fluorescein isothiocyanate (FITC), R-phycoerythrin (R-PE), BD Cy-Chrome™, and PerCP, can be used with single-laser flow cytometers equipped with an argon-ion laser emitting
Fluorochrome Absorption and Emission
to monoclonal antibodies and other proteins. Four of these fluorochromes, fluorescein isothiocyanate (FITC), R-phycoerythrin (R-PE), BD Cy-Chrome™, and PerCP, can be used with single-laser flow cytometers equipped with an argon-ion laser emitting light at 488 nm
The OP9-DL1 System: Generation of T-Lymphocytes from Embryonic or Hematopoietic Stem Cells In Vitro
reagent; BD Biosciences 555899) (for Protocol 4) Buffer for cell staining and cell sorting (for Protocol 4; see Step 72) Complement, reconstituted from rabbit (Cedarlane CL3331) (for Protocol 3) ESC medium (for Protocols 1, 2) Freezing
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