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100ul
| 产品编号 | bs-0648R-FITC |
| 英文名称 | CD8 Rabbit pAb, FITC conjugated |
| 中文名称 | FITC标记的CD8抗体 |
| 英文别名 | CD8 antigen, alpha polypeptide(p32); CD8a; CD8a antigen; CD8a molecule; CD8A_MOUSE; CD8A_HUMAN; Leu2; Leu2 T lymphocyte antigen; MAL; OKT8 T cell antigen; p32; T cell antigen Leu2; T cell co receptor; T-cell surface glycoprotein CD8 alpha chain; T-lymphocyte differentiation antigen T8/Leu-2; T8 T cell antigen. |
| 产品应用 | Flow-Cyt=1ug/test Not yet tested in other applications. |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from mouse CD8 |
| 亚型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Cancer > Tumor immunology > CD markers Immunology > Adaptive Immunity > T Cells > CD Immunology > Adaptive Immunity > T Cells > Cytotoxic Cells |
| 亚基 | In general heterodimer of an alpha and a beta chain linked by two disulfide bonds. Can also form homodimers. |
| 亚细胞定位 | Membrane; Single-pass type I membrane protein. |
| 组织特异性 | Most prominently found in the gastrointestinal tract, skin, lung, and brain but not in liver. |
| 相似性 | Contains 1 Ig-like V-type (immunoglobulin-like) domain. |
| 功能 | Identifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing. CD8 alpha chains binds to class I MHC molecules alpha-3 domains. |
| 保存条件 | Shipped at 4℃. Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell interactions within the immune system. The CD8 antigen acts as a coreceptor with the T-cell receptor on the T lymphocyte to recognize antigens displayed by an antigen presenting cell in the context of class I MHC molecules. The coreceptor functions as either a homodimer composed of two alpha chains or as a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains. This gene encodes the CD8 alpha chain. Multiple transcript variants encoding different isoforms have been found for this gene. The major protein isoforms of this gene differ by the presence or absence of a transmembrane domain and thus differ in being a membrane-anchored or secreted protein. [provided by RefSeq, May 2020] |
| 应用 | 推荐稀释比例 |
| {Flow-Cyt} | {1ug/test} |
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文献和实验KitCD2-PECD3-FITC/PE/APCCD4-FITC/PE/APCCD6-FITC/PE/APCCD8-FITC/PE/APCCD25-Biotin/PECD30-PECD62L-FITC/PE/APCAnti-CRTH-2- Biotin/PEAnti-TCRα/β-PE/APC细胞因子分泌细胞IFN-r Secretion Assay Cell Enrichment and Detection Kit(PE)IL-2 Secretion Assay Cell Enrichment
CXCR5和Ly108（SLAMF6），部分还高表达CD62L；过渡性耗竭T细胞（TEX-Trans）为CD101−TIM3+；终末耗竭T细胞（TEX-Term）则表现为CD101+TIM3+CD69+；而效应样耗竭T细胞（eff-like TEX）高表达KLRG1和CX3CR1。相比之下，在急性感染中，效应T细胞（TEFF）为KLRG1hiCD127lo，记忆前体细胞（TMP）为KLRG1loCD127hi。记忆T细胞进一步分为：干细胞样记忆（TSCM，CD62L+CD45RA+CCR7+IL-7R
Fluorochrome Absorption and Emission Spectra
conjugates to monoclonal antibodies and other proteins. Four of these fluorochromes, fluorescein isothiocyanate (FITC), R-phycoerythrin (R-PE), BD Cy-Chrome™, and PerCP, can be used with single-laser flow cytometers equipped with an argon-ion laser emitting
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