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- bsm-30094M-PE
- 2025年10月15日
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- 规格：
25T/50T/100T
| 规格： | 25T | 产品价格： | ￥220.0 |
|---|---|---|---|
| 规格： | 50T | 产品价格： | ￥360.0 |
| 规格： | 100T | 产品价格： | ￥580.0 |
| 产品编号 | bsm-30094M-PE |
| 英文名称 | human CD8 Mouse mAb, PE conjugated |
| 中文名称 | PE标记的小鼠抗人CD8单克隆抗体 |
| 英文别名 | CD8 antigen, alpha polypeptide(p32); CD8a; CD8a antigen; CD8a molecule; CD8A_MOUSE; CD8A_HUMAN; Leu2; Leu2 T lymphocyte antigen; MAL; OKT8 T cell antigen; p32; T cell antigen Leu2; T cell co receptor; T-cell surface glycoprotein CD8 alpha chain; T-lymphocyte differentiation antigen T8/Leu-2; T8 T cell antigen. |
| 产品应用 | Flow-Cyt=10ul/Test Not yet tested in other applications. |
| 抗体来源 | Mouse |
| 免疫原 | Recombinant human CD8 |
| 亚型 | Mouse IgG1, k |
| 纯化方法 | affinity purified by Protein G |
| 克隆类型 | Monoclonal |
| 储存液 | 0.01M PBS (pH7.4), 0.5% BSA, 0.02% Proclin300. |
| 研究领域 | Cancer > Tumor immunology > CD markers Immunology > Adaptive Immunity > T Cells > CD Immunology > Adaptive Immunity > T Cells > Cytotoxic Cells |
| 亚基 | In general heterodimer of an alpha and a beta chain linked by two disulfide bonds. Can also form homodimers. |
| 亚细胞定位 | Membrane; Single-pass type I membrane protein. |
| 组织特异性 | Most prominently found in the gastrointestinal tract, skin, lung, and brain but not in liver. |
| 相似性 | Contains 1 Ig-like V-type (immunoglobulin-like) domain. |
| 功能 | Identifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing. CD8 alpha chains binds to class I MHC molecules alpha-3 domains. |
| 保存条件 | Store at 2-8℃. Protect from light. Avoid freezing. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell interactions within the immune system. The CD8 antigen acts as a coreceptor with the T-cell receptor on the T lymphocyte to recognize antigens displayed by an antigen presenting cell in the context of class I MHC molecules. The coreceptor functions as either a homodimer composed of two alpha chains or as a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains. This gene encodes the CD8 alpha chain. Multiple transcript variants encoding different isoforms have been found for this gene. The major protein isoforms of this gene differ by the presence or absence of a transmembrane domain and thus differ in being a membrane-anchored or secreted protein. [provided by RefSeq, May 2020] |
| 应用 | 推荐稀释比例 |
| {Flow-Cyt} | {10ul/Test} |
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文献和实验T-cell dependent antigen specific in situ staining (Pe)
and then apply 3% human serum as a blocking agent (health hazard!). 4- incubate with the blocking for 10 min. 5- blot the slides without washing and apply Phycoerythrin (B-Pe or R-Pe) 0.5µg/ml in PBS-BSA-NaN 3 , in a moist chamber, at RT for 1-18 hr.
MHC I STREPTAMERS® 检测与分离抗原特异性 (CD8+ T) 细胞
体，实现了对抗原特异性 (CD8+ T) 细胞的检测和分离 (如，流式分析) 。此技术的特点在于，荧光或磁性微珠等标记物可以通过使用 Biotin (生物素) 而去除，保留了细胞的真实特性，得到的功能性的，label-free 的细胞可用于下游的实验研究。 1) MHC I STREPTAMERS® 技术解析 MHC I STREPTAMERS® 由两部分组成： · MHC I-Strep，即连接了 Twin-Strep-tag® 和抗原肽的 MHC I 分子。 · 荧光 (PE/APC)
Immunofluorescent Staining of Mouse and Rat Leukocytes
, or fluorochrome-conjugated mAbs) to predetermined optimal concentrations (see Staining Tips) in wash buffer and deliver to the wells of a U-bottom microtiter plate in a volume of 50 µl. Deliver 106 cells in 50 µl to each well already containing 50 µl of mAb
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