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- Bioss
- bs-22173R-HRP
- 2025年09月30日
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100ul
| 产品编号 | bs-22173R-HRP |
| 英文名称 | PINK1 Rabbit pAb, HRP conjugated |
| 中文名称 | HRP标记的丝氨酸/苏氨酸蛋白激酶PINK1抗体 |
| 英文别名 | PINK1_HUMAN; PTEN Induced Putative Kinase 1; PTEN-Induced Putative Kinase Protein 1; BRPK; Serine/Threonine-Protein Kinase PINK1, Mitochondrial; Parkinson Disease(Autosomal Recessive) 6; Protein Kinase BRPK; EC 2.7.11.1; PARK6; |
| 产品应用 | WB=1:500-2000 Not yet tested in other applications. |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human PINK1 |
| 亚型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Cardiovascular > Heart > Cardiac metabolism Metabolism > Pathways and Processes > Mitochondrial Metabolism > Mitochondrial markers Metabolism > Pathways and Processes > Mitochondrial Metabolism > Mitophagy fission and fusion Metabolism > Types of disease > Neurodegenerative disease Neuroscience > Neurology process > Neurodegenerative disease > Parkinson's disease Signal Transduction > Metabolism > Mitochondrial Signal Transduction > Protein Phosphorylation > Ser / Thr Kinases > Other Kinases |
| 亚基 | Interacts with PRKN. Interacts with FBXO7. Forms a complex with PRKN and PARK7. |
| 亚细胞定位 | Localizes mostly in mitochondrion and the 2 proteolytic processed fragments of 55 kDa and 48 kDa localize mainly in cytosol. |
| 组织特异性 | Highly expressed in heart, skeletal muscle and testis, and at lower levels in brain, placenta, liver, kidney, pancreas, prostate, ovary and small intestine. Present in the embryonic testis from an early stage of development. |
| 翻译后修饰 | Autophosphorylation at Ser-228 and Ser-402 is essential for Parkin/PRKN recruitment to depolarized mitochondria. _x000D_ Two shorter forms of 55 kDa and 48 kDa seem to be produced by proteolytic cleavage and localize mainly in cytosol. |
| 相似性 | Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. |
| 功能 | Protects against mitochondrial dysfunction during cellular stress by phosphorylating mitochondrial proteins. Involved in the clearance of damaged mitochondria via selective autophagy (mitophagy) by mediating activation and translocation of PRKN . Targets PRKN to dysfunctional depolarized mitochondria through the phosphorylation of MFN2 . Activates PRKN in 2 steps: (1) by mediating phosphorylation at 'Ser-65' of PRKN and (2) mediating phosphorylation of ubiquitin, converting PRKN to its fully-active form . Required for ubiquinone reduction by mitochondrial complex I by mediating phosphorylation of complex I subunit NDUFA10 (By similarity). |
| 保存条件 | Shipped at 4℃. Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | This gene encodes a serine/threonine protein kinase that localizes to mitochondria. It is thought to protect cells from stress-induced mitochondrial dysfunction. Mutations in this gene cause one form of autosomal recessive early-onset Parkinson disease. [provided by RefSeq, Jul 2008] |
| 应用 | 推荐稀释比例 |
| {WB} | {1:500-2000} |
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文献和实验affinity chromatography and conjugated with horseradish peroxidase (HRP). Formulation Supplied as a 0.2 μm filtered solution in phosphate-buffered saline (PBS) (pH 7.0) with stabilizers. Storage Neat samples can be stored at 2° - 8° C
Western Immunoblotting of Proteins
. For a mouse monoclonal antibody, a goat-anti-mouse HRP conjugated antibody is recommended, for a rabbit polyclonal antibody, a goat-anti-rabbit HRP conjugated antibody is advisable. 7. Wash the nitrocellulose membrane five times for 3 to 5 minutes
Western Immunoblotting of Proteins
antibody, a goat-anti-mouse HRP conjugated antibody is recommended, for a rabbit polyclonal antibody, a goat-anti-rabbit HRP conjugated antibody is advisable. 7. Wash the nitrocellulose membrane five times for 3 to 5 minutes each with either water
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