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- bsm-0917M-Cy5
- 2026年10月14日
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100ul
| 产品编号 | bsm-0917M-Cy5 |
| 英文名称 | BrdU(Proliferation Marker) Mouse mAb, Cy5 conjugated |
| 中文名称 | Cy5标记的5-溴脱氧尿嘧啶核苷（增殖标志物）单克隆抗体 |
| 英文别名 | Bromodeoxyuridine; Bromodeoxyuridine; 5-Bromo-2′-deoxyuridine; 5-BrdU; Proliferation Marker; 5-Bromo-2-deoxyUridine. |
| 产品应用 | ICC/IF=1:50-200 Not yet tested in other applications. |
| 抗体来源 | Mouse |
| 免疫原 | KLH conjugated Brdu |
| 亚型 | IgG |
| 纯化方法 | affinity purified by Protein G |
| 克隆类型 | Monoclonal |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Cell Biology > Cell Cycle > Markers Epigenetics and Nuclear Signaling > DNA / RNA > DNA / Nucleotides Neuroscience > Cell Type Marker > Neuron marker > Soma marker Tags & Cell Markers > Cell Type Markers > Replication |
| 亚细胞定位 | Nuclear. |
| 保存条件 | Shipped at 4℃. Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | The immunocytochemical detection of bromodeoxyuridine (BrdU) incorporated into DNA is a powerful tool to study the cytokinetics of normal and neoplastic cells. In vitro or in vivo labeling of tumor cells with the thymidine analogue BrdU and the subsequent detection of incorporated BrdU with specific anti-BrdU monoclonal antibodies is an accurate and comprehensive method to quantitate the degree of DNA-synthesis.BrdU is incorporated into the newly synthezised DNA of S-phase cells may provide an estimate for the fraction of cells in S-phase. Also dynamic proliferative information such as the S-phase transit rate and the potential doubling time can be obtained, by means of bivariate BrdU/DNA flow cytometric analysis |
| 应用 | 推荐稀释比例 |
| {ICC/IF} | {1:50-200} |
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文献和实验Methylation Microarray以及Mouse CpG Island Microarray)，过去Roche NimbleGen也有相应的产品，但从今年下半年已经停产。 使用不同的实验平台，操作方法也不同，illumia芯片前期处理采用的是亚硫酸盐处理，将甲基化的差异转变成碱基上的差异，然后利用两个位点特异的探针 检测这些化学上差异的位点，一个探针是为甲基化位点(M磁珠类型)设计的，而另一个是为未甲基化位点(U磁珠类型)设计的，结合后通过单碱基延伸掺入一个标记了的ddNTP，通过计算甲基
) were in contact with the sections for 90 min at room temperature. This was followed by three washes with PBS and the application of the secondary antibodies (antirabbit IgG-Cy3 or anti-mouse IgM-Cy5) for 45 min at room temperature. After being washed
Fluorochrome Absorption and Emission Spectra
. Due to its photobleaching characteristics, PerCP conjugates are not recommended for use on stream-in-air flow cytometers. PerCP-Cy5.5 is a tandem conjugate system than combines PerCP with a cyanine dye (Cy5.5™) and has an absorption maximum
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