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- 应用范围:
产品信息以Bioss网站为准
- 规格:
100ul
| 产品编号 | bs-19811R-HRP |
| 英文名称 | SLC25A25 Rabbit pAb, HRP conjugated |
| 中文名称 | HRP标记的溶质载体家族蛋白25成员A25抗体 |
| 英文别名 | Calcium-binding mitochondrial carrier protein SCaMC-2; KIAA1896; MCSC; MGC105138; MGC119514; MGC119515; MGC119516; MGC119517; Mitochondrial ATP Mg/Pi carrier; Mitochondrial ATP-Mg/Pi carrier protein 3; Mitochondrial Ca(2+)-dependent solute carrier protein 3; Mitochondrial Ca2+dependent solute carrier; Mitochondrial calcium-dependent solute carrier; PCSCL; RP11 395P17.4; SCAMC 2; SCAMC2; SCMC2_HUMAN; Short calcium binding mitochondrial carrier 2; SLC25A25; Small calcium binding mitochondrial carrier 2; Small calcium-binding mitochondrial carrier protein 2; Solute carrier family 25(mitochondrial carrier phosphate carrier) member 25; Solute carrier family 25 member 25; solute carrier family 25, member 25, isoform a. |
| 产品应用 | WB=1:500-2000 Not yet tested in other applications. |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human SLC25A25 |
| 亚型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Metabolism > Pathways and Processes > Mitochondrial Metabolism > Mitochondrial markers Signal Transduction > Metabolism > Mitochondrial |
| 亚细胞定位 | Mitochondrion inner membrane. |
| 组织特异性 | Present in various cell lines (at protein level). Widely expressed. Expressed in fetal and adult liver, skeletal muscle, testis, ovary, hippocampus and caudate nucleus. Isoform 1 is present in all tissues tested. Isoform 2 expression is restricted to kidney and lung. |
| 相似性 | Belongs to the mitochondrial carrier family. _x000D_ Contains 3 EF-hand domains. _x000D_ Contains 3 Solcar repeats. |
| 功能 | Calcium-dependent mitochondrial solute carrier. Mitochondrial solute carriers shuttle metabolites, nucleotides, and cofactors through the mitochondrial inner membrane. May act as a ATP-Mg/Pi exchanger that mediates the transport of Mg-ATP in exchange for phosphate, catalyzing the net uptake or efflux of adenine nucleotides into or from the mitochondria. |
| 保存条件 | Shipped at 4℃. Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | The protein encoded by this gene belongs to the family of calcium-binding mitochondrial carriers, with a characteristic mitochondrial carrier domain at the C-terminus. These proteins are found in the inner membranes of mitochondria, and function as transport proteins. They shuttle metabolites, nucleotides and cofactors through the mitochondrial membrane and thereby connect and/or regulate cytoplasm and matrix functions. This protein may function as an ATP-Mg/Pi carrier that mediates the transport of Mg-ATP in exchange for phosphate, and likely responsible for the net uptake or efflux of adenine nucleotides into or from the mitochondria. Alternatively spliced transcript variants encoding different isoforms with a common C-terminus but variable N-termini have been described for this gene. [provided by RefSeq, Jul 2012] |
| 应用 | 推荐稀释比例 |
| {WB} | {1:500-2000} |
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文献和实验affinity chromatography and conjugated with horseradish peroxidase (HRP). Formulation Supplied as a 0.2 μm filtered solution in phosphate-buffered saline (PBS) (pH 7.0) with stabilizers. Storage Neat samples can be stored at 2° - 8° C
Western Immunoblotting of Proteins
. For a mouse monoclonal antibody, a goat-anti-mouse HRP conjugated antibody is recommended, for a rabbit polyclonal antibody, a goat-anti-rabbit HRP conjugated antibody is advisable. 7. Wash the nitrocellulose membrane five times for 3 to 5 minutes
Western Immunoblotting of Proteins
antibody, a goat-anti-mouse HRP conjugated antibody is recommended, for a rabbit polyclonal antibody, a goat-anti-rabbit HRP conjugated antibody is advisable. 7. Wash the nitrocellulose membrane five times for 3 to 5 minutes each with either water
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