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MBP tag Mouse mAb, HRP conjuga

ted(bsm-33008M-HRP)-100ul
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  • ￥1200
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  • bsm-33008M-HRP
  • 2025年10月15日
  • 产品信息以Bioss网站为准
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北京博奥森生物技术有限公司
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      100ul

    产品编号bsm-33008M-HRP
    英文名称MBP tag Mouse mAb, HRP conjugated
    中文名称HRP标记的麦芽糖结合蛋白单克隆抗体
    英文别名MBP-tag; MBP; MMBP; Maltose Binding Protein; Maltose binding protein tag; ECK4026; JW3994; Mal E; MalE; malJ; Maltodextrin binding protein; Maltose ABC transporter periplasmic protein; Maltose binding periplasmic protein; Periplasmic maltose binding protein; MALE_ECOLI.
    产品应用WB=1:1000-5000

    Not yet tested in other applications.
    Optimal working dilutions must be determined by the end user.

    抗体来源Mouse
    免疫原Recombinant MBP Protein of Escherichia coli (strain K12)
    亚型IgG
    纯化方法affinity purified by Protein A
    克隆类型Monoclonal
    浓度1mg/ml
    储存液0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
    亚细胞定位Periplasm.
    相似性Belongs to the bacterial solute-binding protein 1 family.
    功能Maltose binding protein-MBP is E. coli malE gene encoding the protein, bacterial member of the maltose transport system. malE gene product can integrate with a variety of proteins, it is an effective medium for expression studies. subunit antibody.
    保存条件Shipped at 4℃. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
    注意事项This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
    背景资料Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. Maltose binding protein(MBP) is the 370 amino acid product of the E.coli mal E gene. MBP is a useful affinity tag that can increase the expression level and solubility of the resulting tagged protein. The MBP tag also promotes proper folding of the attached protein. Plasmid vectors have been constructed utilizing the MBP domain that allow the synthesis of high levels of MBP-fusion proteins that can be purified in a one step procedure by affinity chromatography cross linked amylose resin. Once bound to amylose, the MBP protein can then be separated from the target protein by cleavage by coagulation Factor Xa at a specific four residue site. Alternatively, the intact fusion protein can be specifically eluted from the resin by the addition of excess free maltose. Subsequent to elution, MBP fusion protein can be visualized either by Western blot analysis or immunoprecipitation using antibodies specific for the MBP-tag. An antibody to MBP can also be used to isolate or detect expression of the protein.

     

    应用推荐稀释比例
    {WB}{1:1000-5000}

     

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