Blank control: U2OS.
Primary Antibody (green line): Rabbit Anti-VIM antibody (bs-0756R-FITC)
Dilution: 2μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with PBST for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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- bs-0756R-FITC
- 2025年10月15日
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产品信息以Bioss网站为准
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100ul
| 产品编号 | bs-0756R-FITC |
| 英文名称 | Vimentin Rabbit pAb, FITC conjugated |
| 中文名称 | FITC标记的波形蛋白抗体 |
| 英文别名 | FLJ36605; OTTHUMP00000019224; VIM; VIME_HUMAN; Vimentin. |
| 产品应用 | Flow-Cyt=1μg/Test, IF=1:100-500, ICC/IF=1:100-500 Not yet tested in other applications. |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human Vimentin |
| 亚型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Cancer > Tumor biomarkers Developmental Biology > Lineage specification > Ectoderm Neuroscience > Cell Type Marker > Neural Stem Cell marker Neuroscience > Neurology process > Neurogenesis Neuroscience > Neurology process > Neuroregeneration > Neuroregeneration Signal Transduction > Antibodies > vimentin Signal Transduction > Cytoskeleton / ECM > Cytoskeleton > Intermediate Filaments > Class III > Vimentin Stem Cells > Lineage Markers > Endoderm Stem Cells > Neural Stem Cells > Intracellular |
| 亚基 | Homopolymer assembled from elementary dimers. Interacts with HCV core protein. Interacts with LGSN and SYNM. Interacts (via rod region) with PLEC (via CH 1 domain) (By similarity). Interacts with SLC6A4. Interacts with STK33. Interacts with LARP6. Interacts with RAB8B (By similarity). |
| 亚细胞定位 | Cytoplasm. |
| 组织特异性 | Highly expressed in fibroblasts, somev expression in T- and B-lymphocytes, and little or no expression in Burkitt's lymphoma cell lines. Expressed in many hormone-independent mammary carcinoma cell lines. |
| 翻译后修饰 | Filament disassembly during mitosis is promoted by phosphorylation at Ser-55 as well as by nestin (By similarity). One of the most prominent phosphoproteins in various cells of mesenchymal origin. Phosphorylation is enhanced during cell division, at which time vimentin filaments are significantly reorganized. Phosphorylation by PKN1 inhibits the formation of filaments. Phosphorylated at Ser-56 by CDK5 during neutrophil secretion in the cytoplasm. Phosphorylated by STK33. |
| 相似性 | Belongs to the intermediate filament family. |
| 功能 | Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally. Involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2. |
| 保存条件 | Shipped at 4℃. Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | This gene encodes a member of the intermediate filament family. Intermediate filamentents, along with microtubules and actin microfilaments, make up the cytoskeleton. The protein encoded by this gene is responsible for maintaining cell shape, integrity of the cytoplasm, and stabilizing cytoskeletal interactions. It is also involved in the immune response, and controls the transport of low-density lipoprotein (LDL)-derived cholesterol from a lysosome to the site of esterification. It functions as an organizer of a number of critical proteins involved in attachment, migration, and cell signaling. Mutations in this gene causes a dominant, pulverulent cataract.[provided by RefSeq, Jun 2009] |
| 应用 | 推荐稀释比例 |
| {Flow-Cyt} | {1μg/Test} |
| {IF} | {1:100-500} |
| {ICC/IF} | {1:100-500} |
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文献和实验该产品被引用文献
[IF={{ 5.3 }}] {Sorokina Margarita. et al. Fibro-adipogenic progenitor cells in skeletal muscle unloading: metabolic and functional impairments. SKELET MUSCLE. 2024 Dec;14(1):1-17} {FC} {Rat}
[IF={{ 3.078 }}] {Carolin Krziminski. et al. Development of a bioreactor system for pre‐endothelialized cardiac patch generation with enhanced viscoelastic properties by combined collagen I compression and stromal cell culture. J Tissue Eng Regen M. 2020 Dec;14(12):1749-1762} {FCM} {Human}
相关实验
Fluorochrome Absorption and Emission Spectra
conjugates to monoclonal antibodies and other proteins. Four of these fluorochromes, fluorescein isothiocyanate (FITC), R-phycoerythrin (R-PE), BD Cy-Chrome™, and PerCP, can be used with single-laser flow cytometers equipped with an argon-ion laser emitting
Fluorochrome Absorption and Emission
to monoclonal antibodies and other proteins. Four of these fluorochromes, fluorescein isothiocyanate (FITC), R-phycoerythrin (R-PE), BD Cy-Chrome™, and PerCP, can be used with single-laser flow cytometers equipped with an argon-ion laser emitting light at 488 nm
-1 等； · 间质标志物：Vimentin、α-SMA 等。 具体纯度要求应根据后续实验目的和检测方法确定，不宜采用统一的固定阈值。 3. 功能验证 形成连续单层后，可通过跨上皮电阻（TEER）评价离子屏障形成情况，并结合 FITC-dextran 等通透性实验及 ZO-1 等紧密连接标志物，综合评价上皮屏障功能。 TEER 检测原理 四、高频问题与解决方案 1. 细胞活力差、贴壁率低 · 常见原因：取材处理时间过长、解离过度、培养板包被效果差、培养体系不适合； · 解决
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Vimentin Rabbit pAb, FITC conjugated(bs-0756R-FITC)-100ul
￥2980
