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- Bioss
- bs-4509R-HRP
- 2025年09月30日
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100ul
| 产品编号 | bs-4509R-HRP |
| 英文名称 | CXCL13/BCA1 Rabbit pAb, HRP conjugated |
| 中文名称 | HRP标记的B-淋巴细胞趋化因子抗体 |
| 英文别名 | C-X-C motif chemokine 13; Angie; ANGIE; ANGIE2; B cell-attracting chemokine 1; B cell attracting chemokine 1; B lymphocyte chemoattractant; B-cell chemoattractant; B-cell-attracting chemokine 1; B-cell-homing chemokine(ligand for Burkitt's lymphoma receptor-1); CXL13_MOUSE; BCA-1; BLC; BLR1L; Chemokine(C-X-C motif) ligand 13; Chemokine(C-X-C motif) ligand 13(B-cell chemoattractant); Chemokine, CXC motif, ligand 13; CXC chemokine BLC; CXCL13; SCYB13; Small inducible cytokine B subfamily(Cys-X-Cys motif), member 13(B-cell chemoattractant); Small-inducible cytokine B13; Small inducible cytokine B13; Small inducible cytokine subfamily B, member 13. |
| 产品应用 | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500 Not yet tested in other applications. |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from mouse CXCL13/BCA1 |
| 亚型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Immunology > Innate Immunity > Chemokines > Alpha Chemokines (CXC) |
| 亚细胞定位 | Secreted. |
| 组织特异性 | Highest levels in liver, followed by spleen, lymph node, appendix and stomach. Low levels in salivary gland, mammary gland and fetal spleen. |
| 相似性 | Belongs to the intercrine alpha (chemokine CxC) family. |
| 功能 | Chemotactic for B-lymphocytes but not for T-lymphocytes, monocytes and neutrophils. Does not induce calcium release in B-lymphocytes. Binds to BLR1/CXCR5. |
| 保存条件 | Shipped at 4℃. Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | B lymphocyte chemoattractant, independently cloned and named Angie, is a CXC chemokine strongly expressed in the follicles of the spleen, lymph nodes, and Peyer's patches. It preferentially promotes the migration of B lymphocytes (compared to T cells and macrophages), apparently by stimulating calcium influx into, and chemotaxis of, cells expressing Burkitt's lymphoma receptor 1 (BLR1). It may therefore function in the homing of B lymphocytes to follicles. |
| 应用 | 推荐稀释比例 |
| {WB} | {1:500-2000} |
| {IHC-P} | {1:100-500} |
| {IHC-F} | {1:100-500} |
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文献和实验affinity chromatography and conjugated with horseradish peroxidase (HRP). Formulation Supplied as a 0.2 μm filtered solution in phosphate-buffered saline (PBS) (pH 7.0) with stabilizers. Storage Neat samples can be stored at 2° - 8° C
Western Immunoblotting of Proteins
. For a mouse monoclonal antibody, a goat-anti-mouse HRP conjugated antibody is recommended, for a rabbit polyclonal antibody, a goat-anti-rabbit HRP conjugated antibody is advisable. 7. Wash the nitrocellulose membrane five times for 3 to 5 minutes
Western Immunoblotting of Proteins
antibody, a goat-anti-mouse HRP conjugated antibody is recommended, for a rabbit polyclonal antibody, a goat-anti-rabbit HRP conjugated antibody is advisable. 7. Wash the nitrocellulose membrane five times for 3 to 5 minutes each with either water
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