Blank control: mouse spleen cells(blue).
Primary Antibody: Rabbit Anti- ID1/FITC Conjugated antibody (bs-3529R /FITC, green line), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA;
Isotype Control Antibody: Rabbit IgG/FITC(orange) ,used under the same conditions.
_x000D_
_x000D_
Protocol
_x000D_
The cells were fixed with 4% Paraformaldehyde (10 min) and then permeabilized with ice-cold 90% methanol for 30 min on ice. The cells were washed twice with 1 X PBS. The cells were incubated in 1 X PBS containing 0.5% BSA + 1 0% goat serum (15 min) to block non-specific protein-protein interactions followed by the incubated with antibody (bs-3529R/FITC, 1μg /1x10^6 cells) for 30 min on ice. Acquisition of 20,000 events was performed._x000D_
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- Bioss
- bs-3529R-FITC
- 2025年09月30日
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100ul
| 产品编号 | bs-3529R-FITC |
| 英文名称 | ID1 Rabbit pAb, FITC conjugated |
| 中文名称 | FITC标记的DNA结合抑制因子1抗体 |
| 英文别名 | bHLHb24; Class B basic helix-loop-helix protein 24; DNA binding protein inhibitor ID 1; DNA binding protein inhibitor ID1; DNA-binding protein inhibitor ID-1; Dominant negative helix loop helix protein; ID 1; ID; ID1; ID1_HUMAN; Inhibitor of Differentiation 1; Inhibitor of DNA binding 1. |
| 产品应用 | Flow-Cyt=1ug/Test, ICC/IF=1:100-500 Not yet tested in other applications. |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human ID1 |
| 亚型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Cancer > Invasion/microenvironment > Angiogenesis Cancer > Signal transduction Epigenetics and Nuclear Signaling > Transcription > Cancer susceptibility > Proto-oncogenes Epigenetics and Nuclear Signaling > Transcription > Domain Families > HLH / Leucine Zipper > HLH |
| 亚基 | Heterodimer with other HLH proteins. Interacts with COPS5. |
| 亚细胞定位 | Cytoplasm. Nucleus. |
| 相似性 | Contains 1 bHLH (basic helix-loop-helix) domain. |
| 功能 | ID (inhibitor of DNA binding) HLH proteins lack a basic DNA-binding domain but are able to form heterodimers with other HLH proteins, thereby inhibiting DNA binding. |
| 保存条件 | Shipped at 4℃. Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | ID proteins contain a helix-loop-helix (HLH) motif and regulate tissue-specific transcription within several cell lineages. They do not bind DNA directly, but inhibit lineage commitment by binding basic helix-loop-helix (bHLH) transcription factors through their HLH motif. ID proteins contribute to cell growth, senescence, differentiation and angiogenesis. Id1 mRNA is highly expressed in heart, lung and kidney and has lower expression in brain and liver. Two transcript variants encoding different isoforms have been found for this gene. |
| 应用 | 推荐稀释比例 |
| {Flow-Cyt} | {1ug/Test} |
| {ICC/IF} | {1:100-500} |
Blank control: mouse kidney cells(blue).
_x000D_ Primary Antibody: Rabbit Anti- ID1/FITC Conjugated antibody (bs-3529R /FITC, green line), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA;
_x000D_ Isotype Control Antibody: Rabbit IgG/FITC(orange) ,used under the same conditions.
_x000D_ Protocol
_x000D_ The cells were fixed with 4% Paraformaldehyde (10 min) and then permeabilized with ice-cold 90% methanol for 30 min on ice. The cells were washed twice with 1 X PBS. The cells were incubated in 1 X PBS containing 0.5% BSA + 1 0% goat serum (15 min) to block non-specific protein-protein interactions followed by the incubated with antibody (bs-3529R/FITC, 1μg /1x10^6 cells) for 30 min on ice. Acquisition of 20,000 events was performed.
_x000D_ Primary Antibody: Rabbit Anti- ID1/FITC Conjugated antibody (bs-3529R /FITC, green line), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA;
_x000D_ Isotype Control Antibody: Rabbit IgG/FITC(orange) ,used under the same conditions.
_x000D_ Protocol
_x000D_ The cells were fixed with 4% Paraformaldehyde (10 min) and then permeabilized with ice-cold 90% methanol for 30 min on ice. The cells were washed twice with 1 X PBS. The cells were incubated in 1 X PBS containing 0.5% BSA + 1 0% goat serum (15 min) to block non-specific protein-protein interactions followed by the incubated with antibody (bs-3529R/FITC, 1μg /1x10^6 cells) for 30 min on ice. Acquisition of 20,000 events was performed.
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Fluorochrome Absorption and Emission Spectra
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Fluorochrome Absorption and Emission
to monoclonal antibodies and other proteins. Four of these fluorochromes, fluorescein isothiocyanate (FITC), R-phycoerythrin (R-PE), BD Cy-Chrome™, and PerCP, can be used with single-laser flow cytometers equipped with an argon-ion laser emitting light at 488 nm
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ID1 Rabbit pAb, FITC conjugated(bs-3529R-FITC)-100ul
￥2980
