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- 详细信息
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- 应用范围:
产品信息以Bioss网站为准
- 规格:
100ul
| 产品编号 | bs-0098R-HRP |
| 英文名称 | GIP Rabbit pAb, HRP conjugated |
| 中文名称 | HRP标记的胃泌素抑制肽抗体 |
| 英文别名 | Gastric Inhibitory polypeptide; Glucose dependent insulinotropic polypeptide; Gastric Inhibitory Peptide; GIP_HUMAN; Glucose-dependent insulinotropic polypeptide; Incretin hormone. |
| 产品应用 | IHC-P=1:100-500, IHC-F=1:100-500 Not yet tested in other applications. |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human GIP |
| 亚型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Signal Transduction > Growth Factors/Hormones > Hormones Signal Transduction > Growth Factors/Hormones > Insulin / Insulin-like Signal Transduction > Signaling Pathway > G Protein Signaling > GPCR |
| 亚细胞定位 | Secreted. |
| 相似性 | Belongs to the glucagon family. |
| 功能 | Potent stimulator of insulin secretion and relatively poor inhibitor of gastric acid secretion. |
| 保存条件 | Shipped at 4℃. Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | bs-0098P is one synthetic peptide derived from human GIP.GIP (Gastric Inhibitory polypeptide ) belongs to the glucagon superfamily. The encoded protein is important in maintaining glucose homeostasis as it is a potent stimulator of insulin secretion from pancreatic beta-cells following food ingestion and nutrient absorption. This gene stimulates insulin secretion via its G protein-coupled receptor activation of adenylyl cyclase and other signal transduction pathways. It is a relatively poor inhibitor of gastric acid secretion. Mature GIP is a highly conserved 42 amino acid polypeptide belonging to the glucagon family. GIP is highly expressed as a pre-pro-protein in the duodenum and the jejunum. The mature secreted polypeptide acts as a potent stimulator of insulin secretion and a poor inhibitor of gastric acid secretion. |
| 应用 | 推荐稀释比例 |
| {IHC-P} | {1:100-500} |
| {IHC-F} | {1:100-500} |
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文献和实验affinity chromatography and conjugated with horseradish peroxidase (HRP). Formulation Supplied as a 0.2 μm filtered solution in phosphate-buffered saline (PBS) (pH 7.0) with stabilizers. Storage Neat samples can be stored at 2° - 8° C
Western Immunoblotting of Proteins
. For a mouse monoclonal antibody, a goat-anti-mouse HRP conjugated antibody is recommended, for a rabbit polyclonal antibody, a goat-anti-rabbit HRP conjugated antibody is advisable. 7. Wash the nitrocellulose membrane five times for 3 to 5 minutes
Western Immunoblotting of Proteins
antibody, a goat-anti-mouse HRP conjugated antibody is recommended, for a rabbit polyclonal antibody, a goat-anti-rabbit HRP conjugated antibody is advisable. 7. Wash the nitrocellulose membrane five times for 3 to 5 minutes each with either water
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