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- 详细信息
- 文献和实验
- 技术资料
- 应用范围:
产品信息以Bioss网站为准
- 规格:
100ul
| 产品编号 | bs-13445R-HRP |
| 英文名称 | G3PP Rabbit pAb, HRP conjugated |
| 中文名称 | HRP标记的转运蛋白G3PP抗体 |
| 英文别名 | glycerol-3-phosphate permease; glycerol-3-phosphate; G-3-P permease; G-3-P transporter; G3P permease; G3P transporter; G3PP; GLPT_HUMAN; Glycerol-3-phosphate permease; Glycerol-3-phosphate transporter; SLC37A1; solute carrier family 37(glycerol-3-phosphate transporter), member 1; solute carrier family 37 member 1. |
| 产品应用 | IHC-P=1:100-500, IHC-F=1:100-500, ELISA=1:5000-10000 Not yet tested in other applications. |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human G3PP/SLC37A1 |
| 亚型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 亚细胞定位 | Membrane. |
| 组织特异性 | Expressed in numerous tissues, with highest expression in kidney, bone marrow, spleen, liver, small intestine, as well as in fetal brain, liver and spleen. |
| 相似性 | Belongs to the major facilitator superfamily. Organophosphate:Pi antiporter (OPA) (TC 2.A.1.4) family. |
| 保存条件 | Shipped at 4℃. Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | G3PP is a 533 amino acid protein belonging to the major facilitator superfamily. G3PP is a multi-pass membrane protein that acts as a transporter of or glycerol-3-phoshphate between cellular compartments. Glycerol-3-phosphate is produced from glycerol and is an important factor in many biochemical pathways, including glycolysis. G3PP contains 12 putative transmembrane domains and N- and C-terminal Endoplasmic Reticulum (ER) retention signals. G3PP is expressed at highest levels in liver, kidney, small intestine, bone marrow and fetal tissues. |
| 应用 | 推荐稀释比例 |
| {IHC-P} | {1:100-500} |
| {IHC-F} | {1:100-500} |
| {ELISA} | {1:5000-10000} |
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文献和实验Immunofluorescence: Rabbit Anti-Murine RELMα
for 25 minutes. 3. Incubate the tissue section with blocking buffer for 20 minutes. 4. Incubate the tissue section overnight at 4?C with Rabbit Anti-Murine RELMα at 0.2 μg/mL in 1X PBS with 0.01% Triton-X and 0.5% BSA. Wash the slide
affinity chromatography and conjugated with horseradish peroxidase (HRP). Formulation Supplied as a 0.2 μm filtered solution in phosphate-buffered saline (PBS) (pH 7.0) with stabilizers. Storage Neat samples can be stored at 2° - 8° C
Western Immunoblotting of Proteins
. For a mouse monoclonal antibody, a goat-anti-mouse HRP conjugated antibody is recommended, for a rabbit polyclonal antibody, a goat-anti-rabbit HRP conjugated antibody is advisable. 7. Wash the nitrocellulose membrane five times for 3 to 5 minutes
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