Blank control:U251.
Primary Antibody (green line): Rabbit Anti-GFAP antibody (bs-0199R-FITC)
Dilution: 2μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 0.1%PBST for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. Acquisition of 20,000 events was performed.
- ￥2980
- Bioss
- bs-0199R-FITC
- 2025年10月15日
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100ul
| 产品编号 | bs-0199R-FITC |
| 英文名称 | GFAP Rabbit pAb, FITC conjugated |
| 中文名称 | FITC标记的胶质纤维酸性蛋白抗体 |
| 英文别名 | Astrocyte Marker; FLJ45472; GFAP; Glial Fibrillary Acidic Protein; Intermediate filament protein; GFAP_HUMAN. |
| 产品应用 | Flow-Cyt=1μg/Test, IF=1:200-800 Not yet tested in other applications. |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human GFAP |
| 亚型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Neuroscience > Cell Adhesion Proteins > Cytoskeletal Proteins > Intermediate Filaments Neuroscience > Cell Type Marker > Glia marker > Astrocyte marker Signal Transduction > Cytoskeleton / ECM > Cytoskeleton > Intermediate Filaments > Class II > GFAP Stem Cells > Neural Stem Cells > Glial Restricted Lineage > Astrocyte Tags & Cell Markers > Cell Type Markers > Neuroscience Markers > Glial |
| 亚基 | Interacts with SYNM. Isoform 3 interacts with PSEN1 (via N-terminus). |
| 亚细胞定位 | Cytoplasm. Note=Associated with intermediate filaments. |
| 组织特异性 | Expressed in cells lacking fibronectin. |
| 翻译后修饰 | Phosphorylated by PKN1. |
| 相似性 | Belongs to the intermediate filament family. |
| 功能 | GFAP, a class-III intermediate filament, is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells. |
| 保存条件 | Shipped at 4℃. Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | This gene encodes one of the major intermediate filament proteins of mature astrocytes. It is used as a marker to distinguish astrocytes from other glial cells during development. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Oct 2008] |
| 应用 | 推荐稀释比例 |
| {Flow-Cyt} | {1μg/Test} |
| {IF} | {1:200-800} |
Blank control: Hela.
Primary Antibody (green line): Rabbit Anti-GFAP antibody (bs-0199R-FITC)
Dilution: 2μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with PBST for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature.Acquisition of 20,000 events was performed.
Primary Antibody (green line): Rabbit Anti-GFAP antibody (bs-0199R-FITC)
Dilution: 2μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with PBST for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature.Acquisition of 20,000 events was performed.
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文献和实验该产品被引用文献
[IF={{ 5.273 }}] {Liya Li. et al. PirB negatively regulates the inflammatory activation of astrocytes in a mouse model of sleep deprivation. NEUROPHARMACOLOGY. 2023 May;:109571} {IF} {Mouse}
[IF={{ 3.41 }}] {Hu, Minghui, et al. "Neuroprotection effect of Interleukin?\17 secreted by reactive astrocytes is emerged from a high?\level IL?\17?\containing environment during acute neuroinflammation."nical & Experimental Immunology (2013).66..} {Other} {="Rat"}
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GFAP Rabbit pAb, FITC conjugated(bs-0199R-FITC)-100ul
￥2980
