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- bs-1000R-AP
- 2026年01月22日
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| 产品编号 | bs-1000R-AP |
| 英文名称 | CNPase Rabbit pAb, AP conjugated |
| 中文名称 | AP标记的2’,3’-环腺苷酸-3’-磷酸二酯酶抗体 |
| 英文别名 | 2', 3'-cyclic-nucleotide 3'-phosphodiesterase; CNPase; Cnp; Cnp-1; Cnp1; 2''; 2'3' cyclic nucleotide 3' phosphodiesterase; 2'3'-cyclic-nucleotide 3'-phosphodiesterase; 3''-cyclic-nucleotide 3''-phosphodiesterase; CN37_HUMAN; CNP 1; CNP; CNP1. |
| 产品应用 | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500 Not yet tested in other applications. |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human CNPase |
| 亚型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Neuroscience > Cell Type Marker > Glia marker > Oligodendrocyte marker Neuroscience > Neurology process > Neurodegenerative disease Tags & Cell Markers > Cell Type Markers > Neuroscience Markers > Glial |
| 亚基 | Exists as monomers and homodimers. |
| 亚细胞定位 | Membrane. Melanosome. Firmly bound to membrane structures of brain white matter. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. |
| 相似性 | Belongs to the cyclic nucleotide phosphodiesterase family. |
| 功能 | May participate in RNA metabolism in the myelinating cell, CNP is the third most abundant protein in central nervous system myelin. |
| 保存条件 | Shipped at 4℃. Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | Predicted to enable 2',3'-cyclic-nucleotide 3'-phosphodiesterase activity. Involved in substantia nigra development. Located in several cellular components, including extracellular space; microtubule; and plasma membrane. Implicated in hypomyelinating leukodystrophy 20; multiple sclerosis; and schizophrenia. Biomarker of alcoholic liver cirrhosis; multiple sclerosis; and restless legs syndrome. [provided by Alliance of Genome Resources, Nov 2021] |
| 应用 | 推荐稀释比例 |
| {WB} | {1:500-2000} |
| {IHC-P} | {1:100-500} |
| {IHC-F} | {1:100-500} |
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文献和实验。下面是来自使用者的反馈数据： 1、蛋白含量低，得到的WB信号弱--SignalBoostTM可以增强信号，检测到低丰度蛋白： 样品：K562细胞全细胞裂解液。 （Lane 1: 25μg总蛋白；Lane 2: 5μg总蛋白；Lane 3: 2.5μg总蛋白） 一抗：anti-PKC（Ab－2）小鼠单抗（MC5）（货号OP74），分别用5%脱脂奶粉PBST溶液（图A）和SignalBoost Solution 1（图B）按照1：1000比例稀释。 二抗：Rabbit anti
SDS-PA gels, and subsequent detection of proteins using rabbit antisera and alkaline phosphatase-conjugated goat-anti-rabbit IgG, detected using bromo-chloro-indolyl phosphate (BCIP) and Nitro-blue tetrazolium (NBT) salts. ・
Developing Genetically Engineered Mouse Models to Study Tumor Suppression
Literature Cited Armstrong, J.F., Kaufman, M.H., Harrison, D.J., and Clarke, A.R. 1995. High‐frequency developmental abnormalities in p53‐deficient mice. Curr
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