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- Bioss
- bs-2531R-HRP
- 2025年12月31日
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100ul
| 产品编号 | bs-2531R-HRP |
| 英文名称 | NES/Kallikrein 10 Rabbit pAb, HRP conjugated |
| 中文名称 | HRP标记的上皮细胞特异性蛋白1抗体 |
| 英文别名 | Kallikrein 10; Breast normal epithelial cell associated serine protease; Kallikrein related peptidase 10; KLK 10; KLK10; KLK10_HUMAN; NES 1; NES1; Normal epithelial cell specific 1; Protease serine like 1; PRSS L1; PRSSL 1; PRSSL1. |
| 产品应用 | WB=1:500-2000 Not yet tested in other applications. |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human KLK10 |
| 亚型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 亚细胞定位 | Secreted. |
| 组织特异性 | Expressed in breast, ovary and prostate. |
| 相似性 | Belongs to the peptidase S1 family. Kallikrein subfamily._x000D_
Contains 1 peptidase S1 domain. |
| 功能 | Has a tumor-suppressor role for NES1 in breast and prostate cancer. |
| 保存条件 | Shipped at 4℃. Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | Kallikrein 10 is a trypsin-like serine proteinase. Originally described from a subtractive library looking for genes that were down-regulated in breast cancer, kallikrein 10 was thought to be epithelial cell specific. Kallikrein 10 is reported to be up regulated in pancreatic cancer and ovarian cancer, but down regulated in testicular cancer and breast cancer, perhaps due to differential hormonal regulation, or tissue specificities. Breast cancer patients that had elevated kallikrein 10 had a worse prognosis, leading to speculation that kallikrein 10 might be protective in breast cancer, similar to the argument put forth for kallikrein 3 in prostate cancer. Kallikrein 10 is found in high levels in the pancreas and in lower levels in the tonsils, esophagus, skin, brain, testis and ovary. Biological fluids (breast milk, serum, seminal plasma, urine) contain kallikrein 10, the highest levels found in milk. The cerebral spinal fluid (CSF) contains Kallikrein 10, and was reported to be elevated in patients with Alzheimer’s disease. Endogenous inhibitors include kallistatin, protein-C inhibitor and 1-proteinase inhibitor, although Kallikrein 10 can be found complexed to a number of different proteinase inhibitors. |
| 应用 | 推荐稀释比例 |
| {WB} | {1:500-2000} |
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文献和实验Western Immunoblotting of Proteins
. For a mouse monoclonal antibody, a goat-anti-mouse HRP conjugated antibody is recommended, for a rabbit polyclonal antibody, a goat-anti-rabbit HRP conjugated antibody is advisable. 7. Wash the nitrocellulose membrane five times for 3 to 5 minutes
Western Immunoblotting of Proteins
antibody, a goat-anti-mouse HRP conjugated antibody is recommended, for a rabbit polyclonal antibody, a goat-anti-rabbit HRP conjugated antibody is advisable. 7. Wash the nitrocellulose membrane five times for 3 to 5 minutes each with either water
. For alkaline phosphatase, use a 1:3000 dilution of Biorad goat anti-mouse or anti-rabbit alkaline phosphatase conjugated antibody in blotto. Incubate on rocker 1 hr at 25℃. 8. Wash 3X3min in TBS/Tween. 9. Alkaline phosphatase development: Add developing
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