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- Bioss
- bs-21423R-HRP
- 2025年12月14日
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100ul
| 产品编号 | bs-21423R-HRP |
| 英文名称 | NOXA2 Rabbit pAb, HRP conjugated |
| 中文名称 | HRP标记的 NADPH氧化酶活化蛋白1抗体 |
| 英文别名 | NCF-2; Ncf2; Neutrophil NADPH oxidase factor 2; p67-phox; p67phox; neutrophil cytosol factor 2 isoform 1; neutrophil cytosolic factor 2(65kD, chronic granulomatous disease, autosomal 2); NADPH oxidase activator 2; neutrophil cytosol factor 2; 67 kDa neutrophil oxidase factor; NCF2_HUMAN. |
| 产品应用 | WB=1:500-2000 Not yet tested in other applications. |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human NOXA2 |
| 亚型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Cancer > Cancer Metabolism > Cellular metabolic process Immunology > Adaptive Immunity > T Cells > Cytotoxic Cells Metabolism > Types of disease > Cancer |
| 亚基 | Component of an NADPH oxidase complex composed of a heterodimer formed by the membrane proteins CYBA and CYBB and the cytosolic subunits NCF1, NCF2 and NCF4. Interacts with NCF4. Interacts (via the C-terminal SH3 domain) with NCF1 (via C-terminus). Interacts with SYTL1 and RAC1. May interact with NOXO1. Interacts with S100A8 and calprotectin (S100A8/9). |
| 亚细胞定位 | Cytoplasm. |
| 相似性 | Belongs to the NCF2/NOXA1 family. {ECO:0000305}._x000D_
Contains 1 PB1 domain._x000D_ Contains 2 SH3 domains._x000D_ Contains 3 TPR repeats. |
| 功能 | NCF2, NCF1, and a membrane bound cytochrome b558 are required for activation of the latent NADPH oxidase (necessary for superoxide production). |
| 保存条件 | Shipped at 4℃. Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | This gene encodes neutrophil cytosolic factor 2, the67-kilodalton cytosolic subunit of the multi-protein NADPH oxidase complex found in neutrophils. This oxidase produces a burst of superoxide which is delivered to the lumen of the neutrophil phagosome. Mutations in this gene, as well as in other NADPH oxidase subunits, can result in chronic granulomatous disease, a disease that causes recurrent infections by catalase-positive organisms. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq]. |
| 应用 | 推荐稀释比例 |
| {WB} | {1:500-2000} |
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文献和实验affinity chromatography and conjugated with horseradish peroxidase (HRP). Formulation Supplied as a 0.2 μm filtered solution in phosphate-buffered saline (PBS) (pH 7.0) with stabilizers. Storage Neat samples can be stored at 2° - 8° C
Western Immunoblotting of Proteins
. For a mouse monoclonal antibody, a goat-anti-mouse HRP conjugated antibody is recommended, for a rabbit polyclonal antibody, a goat-anti-rabbit HRP conjugated antibody is advisable. 7. Wash the nitrocellulose membrane five times for 3 to 5 minutes
Western Immunoblotting of Proteins
antibody, a goat-anti-mouse HRP conjugated antibody is recommended, for a rabbit polyclonal antibody, a goat-anti-rabbit HRP conjugated antibody is advisable. 7. Wash the nitrocellulose membrane five times for 3 to 5 minutes each with either water
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