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- bs-2499R-Cy7
- 2025年11月17日
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100ul
| 产品编号 | bs-2499R-Cy7 |
| 英文名称 | CD69 Rabbit pAb, Cy7 conjugated |
| 中文名称 | Cy7标记的活化诱导分子CD69抗体 |
| 英文别名 | CLEC2C; AIM; Early activation antigen CD69; Early T-cell activation antigen p60; GP32/28; Leukocyte surface antigen Leu-23; MLR-3; EA1; BL-AC/P26; C-type lectin domain family 2 member C; CD69_HUMAN; |
| 产品应用 | Flow-Cyt=1μg/Test Not yet tested in other applications. |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human CD69 |
| 亚型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Immunology > Adaptive Immunity > Regulatory T Cells Immunology > Adaptive Immunity > T Cells > CD Immunology > Cell Type Markers > CD > NK Cells Stem Cells > Hematopoietic Progenitors > Lymphoid > B Lymphocytic Lineage Stem Cells > Hematopoietic Progenitors > Lymphoid > NK Cell Lineage Stem Cells > Hematopoietic Progenitors > Lymphoid > T Lymphocytic Lineage |
| 亚基 | Homodimer; disulfide-linked. |
| 亚细胞定位 | Membrane; Single-pass type II membrane protein. |
| 组织特异性 | Expressed on the surface of activated T-cells, B-cells, natural killer cells, neutrophils, eosinophils, epidermal Langerhans cells and platelets. |
| 翻译后修饰 | Constitutive Ser/Thr phosphorylation in both mature thymocytes and activated T-lymphocytes. |
| 相似性 | Contains 1 C-type lectin domain. |
| 功能 | Involved in lymphocyte proliferation and functions as a signal transmitting receptor in lymphocytes, natural killer (NK) cells, and platelets. |
| 保存条件 | Shipped at 4℃. Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | This gene encodes a member of the calcium dependent lectin superfamily of type II transmembrane receptors. Expression of the encoded protein is induced upon activation of T lymphocytes, and may play a role in proliferation. Furthermore, the protein may act to transmit signals in natural killer cells and platelets. [provided by RefSeq, Aug 2011] |
| 应用 | 推荐稀释比例 |
| {Flow-Cyt} | {1μg/Test} |
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文献和实验Fluorochrome Absorption and Emission Spectra
light at 488 nm for three-color flow cytometric analysis (Fig. 1). BD Biosciences Pharmingen also offers monoclonal antibodies and proteins conjugated to allophycocyanin (APC) and avidin conjugated to Texas Red™. Both of these dyes
Fluorochrome Absorption and Emission
for three-color flow cytometric analysis (Fig. 1). BD Biosciences Pharmingen also offers monoclonal antibodies and proteins conjugated to allophycocyanin (APC) and avidin conjugated to Texas Red™. Both of these dyes are useful in experiments
细胞上表达，因此单独用CD4设门无法将CD4+T细胞区分开来。那么我们用CD3和CD4双参数设门是否可以呢？回答是否定的。因为在佛波酯的刺激作用下CD4 抗原的表达会迅速下调，甚至完全丧失，所以不适合于设门。用CD3和CD8设门，因CD8不受佛波脂的影响且绝大多数CD3+CD8-的细胞都是CD3+CD4+细胞，因此可用CD3+CD8-细胞群来确定CD4+T细胞群。FITC标记CD8，PE标记IL-4和，PE-CY5标记IFN-r，APC或PE-CY7标记CD3。 流式细胞仪要求：488nm或633
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