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100ul
| 产品编号 | bs-1092R-FITC |
| 英文名称 | MRP2/ABCC2 Rabbit pAb, FITC conjugated |
| 中文名称 | FITC标记的多药耐药相关蛋白2抗体 |
| 英文别名 | multidrug resistance-associated protein2; ABC30; ABCC2; ATP binding cassette sub family C(CFTR/MRP) member 2; ATP binding cassette subfamily C member 2; Canalicular multidrug resistance protein; Canalicular multispecific organic anion transporter 1; CMOAT; CMOAT1; cMRP; DJS; KIAA1010; MRP 2; MRP-2; MRP2; Multidrug resistance associated protein 2; MRP2_HUMAN; ATP-binding cassette sub-family C member 2; Multidrug resistance-associated protein 2. |
| 产品应用 | IF=1:100-500, Flow-Cyt=1ug/test Not yet tested in other applications. |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human MRP2 |
| 亚型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Cancer > Drug resistance > MRP-related proteins Signal Transduction > Metabolism > Plasma Membrane > Channels |
| 亚细胞定位 | Membrane; Multi-pass membrane protein. |
| 组织特异性 | Found on the apical membrane of polarized cells in liver, kidney and intestine. The highest expression is found in liver. |
| 相似性 | Belongs to the ABC transporter superfamily. ABCC family. Conjugate transporter (TC 3.A.1.208) subfamily. Contains 2 ABC transmembrane type-1 domains. Contains 2 ABC transporter domains. |
| 功能 | Mediates hepatobiliary excretion of numerous organic anions. May function as a cellular cisplatin transporter. |
| 保存条件 | Shipped at 4℃. Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | multidrug resistance-associated protein 2 is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MRP subfamily which is involved in multi-drug resistance. This protein is expressed in the canalicular (apical) part of the hepatocyte and functions in biliary transport. Substrates include anticancer drugs such as vinblastine; therefore, this protein appears to contribute to drug resistance in mammalian cells. Several different mutations in this gene have been observed in patients with Dubin-Johnson syndrome (DJS), an autosomal recessive disorder characterized by conjugated hyperbilirubinemia. Belongs to the ABC transporter family. |
| 应用 | 推荐稀释比例 |
| {IF} | {1:100-500} |
| {Flow-Cyt} | {1ug/test} |
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文献和实验Fluorochrome Absorption and Emission Spectra
conjugates to monoclonal antibodies and other proteins. Four of these fluorochromes, fluorescein isothiocyanate (FITC), R-phycoerythrin (R-PE), BD Cy-Chrome™, and PerCP, can be used with single-laser flow cytometers equipped with an argon-ion laser emitting
Fluorochrome Absorption and Emission
to monoclonal antibodies and other proteins. Four of these fluorochromes, fluorescein isothiocyanate (FITC), R-phycoerythrin (R-PE), BD Cy-Chrome™, and PerCP, can be used with single-laser flow cytometers equipped with an argon-ion laser emitting light at 488 nm
）和NaveniFlex Tissue Atto647N，带有荧光染料用于Cy 5滤光片组（Abs max：649 nm;Exc 最大 662 nm）。 Q3：NaveniFlex Tissue能否同时阻断细胞特异性背景和自发荧光？ A：不能，只有组织内特定细胞类型产生的非特异性背景被阻断。对于大多数组织，自发荧光的强度远低于PLA信号，因此易于检测。如果您有自发荧光问题，我们建议您选择带有 Atto647N 检测荧光团的 NaveniFlex 组织试剂盒。如果问题仍然存在，则可以在 FITC 通道中识别并减去自发
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