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100ul
| 产品编号 | bs-6577R-AP |
| 英文名称 | S100P binding protein Rabbit pAb, AP conjugated |
| 中文名称 | AP标记的S100P结合蛋白抗体 |
| 英文别名 | S100P-binding protein; S100P-binding protein Riken; y S100PBP; S100PBPR; S1PBP_HUMAN. |
| 产品应用 | WB=1:500-2000 Not yet tested in other applications. |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human S100PBP |
| 亚型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Cancer > Tumor biomarkers Signal Transduction > Signaling Pathway > Calcium Signaling > Calcium Binding Proteins |
| 亚基 | Interacts with S100P. |
| 亚细胞定位 | Nucleus. Note=Colocalizes with S100P in the nucleus. |
| 组织特异性 | Expressed in brain, spleen, and lung. Not detected in pancreas or liver. In pancreas, expressed predominantly in islet cells and to a lesser extent in acinar cells, but not expressed in ductal cells. Up-regulated in various pancreatic ductal adenocarcinomas and pancreatic intraepithelial neoplasias. Detected in pancreatic ductal adenocarcinoma cells (at protein level). Not detected in non-neoplastic ductal epithelium (at protein level). |
| 保存条件 | Shipped at 4℃. Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | S100PBP was originally cloned from a pancreatic epithelioid carcinoma library and encodes a predicted 408 amino acid protein. RT-PCR detected S100PBP expression in brain, breast, spleen, and lung, but not in pancreas and liver. GFP-tagged S100PBP localized to nuclei of transfected HeLa cells. |
| 应用 | 推荐稀释比例 |
| {WB} | {1:500-2000} |
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文献和实验DamIP: Using Mutant DNA Adenine Methyltransferase to Study DNA‐Protein Interactions In Vivo
for studying DNA?protein interaction in vivo. A mutant form of DNA adenine methyltransferase (DamK9A) from E. coli is fused to the protein of interest and expressed. The fusion protein will bind to target binding sites and introduce N 6 ?adenine methylation
Rapid Inactivation of Proteins by Knocksideways
Bayle, J.H., Grimley, J.S., Stankunas, K., Gestwicki, J.E., Wandless, T.J., and Crabtree, G.R. 2006. Rapamycin analogs with differential binding specificity permit orthogonal control of protein activity. Chem
localization microscopywith bimolecular fluorescence complementation (BiFC-PALM) for nanoscale imagingof protein-protein interactions in cells. PLoSOne 9, e100589,doi:10.1371/journal.pone.0100589 (2014).[6]. Rudolf,R., Mongillo, M., Rizzuto, R. & Pozzan, T
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