- 询价
- Signalway Antibody
- C92899FITC
- 2025年09月22日
- FC;ICC;IF
- mouse
- Hu Ms Rat
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- 详细信息
- 文献和实验
- 技术资料
- 形态：
liquid
- 保存条件：
Store at -20˚C
- 克隆性：
Monoclonal
- 适应物种：
Hu Ms Rat
- 保质期：
12 months
- 抗原来源：
mouse
- 供应商：
南京赛戈巍生物科技有限公司
- 宿主：
mouse
- 应用范围：
FC;ICC;IF
- 靶点：
Uniprot：P14635
- 抗体英文名：
Cyclin B1 mouse Monoclonal Antibody FITC Conjugated
- 规格：
50ul/100ul
计算分子量:48
配方:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
应用详情:Flow-Cyt=2ug/Test ICC=1:50-200 IF=1:50-200
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文献和实验after the Antibody has eluted into the column. c) Add 5mL of PBS on top. d)Open Stopcock. collect in 2mL fractions, discarding the first 2mL and keeping the 2nd 2mL fraction. The second 2mL fraction contains the conjugated antibody*. *NOTE: Step d
Immunodetection of cyclin D1 and D2/D3 using 488/630 nm dual laser flow cytometr（图）
. It has been tested with antibodies against cyclin D1 (Pharmingen cat. no. 14561A, clone G12-4326), cyclin D2/D3 (cat. no. 14711A, clone G107-22) and cyclin E, and is based on protocols originally designed by Z. Darzynkiewicz and colleagues. Although FITC-conjugated
Immunodetection of cyclin D1 and D2/D3 using flow cytometry
5-conjugated anti-mouse IgG (available from Jackson ImmunoResearch and Caltag) in a volume of 200 µ l. Incubate for 4 hours at 4°C Wash once with cold cyclin staining buffer and once with cold PBS/azide. Resuspend the cells in propidium iodide at 50 µ g/ml
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