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- 详细信息
- 文献和实验
- 技术资料
- 形态:
liquid
- 保存条件:
Store at -20˚C
- 克隆性:
Monoclonal
- 适应物种:
Human
- 保质期:
12 months
- 抗原来源:
mouse
- 供应商:
南京赛戈巍生物科技有限公司
- 宿主:
mouse
- 应用范围:
WB IHC ICC
- 靶点:
Uniprot:Q15848
- 抗体英文名:
Adiponectin mouse Monoclonal Antibody HRP Conjugated
- 规格:
50ul/100ul
计算分子量:25
配方:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
应用详情:WB=1:500-2000 IHC-P=1:50-200 IHC-F=1:50-200 ICC=1:50-200
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文献和实验Monoclonal Antibody Production Protocol
the rinse twice. Add 100 ul of blocking solution to every well, leave 1 hr at room Temp or O.N at 4°C. PRIMARY ANTIBODY Add the antibody to be tested: Sup of cells = 25 ul, mix well by pipetting up and down (10 times).serum, ascites = 1:100 and a series
Monoclonal Antibody Production
Order 6 six week old Balb/C mice and let the ARC know they are coming.Have your antigen ready for when they arrive.Once they get there earmark the mice and perform a pre-bleed on them to be used as an ELISA control for monitoring the titer
Conjugation of Antibody to HRP
the first peak. Preparation of Ab:HRP Conjugate Immediately mix the HRP with the thiolated antibody at 1 mg HRP per mg antibody. Gently stir for 16-20 hours at 2-8o C
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