- 询价
- Signalway Antibody
- C91854PE-Cy7
- 2025年09月22日
- FC;IF
- Rabbit
- Hu Ms Rat
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- 详细信息
- 文献和实验
- 技术资料
- 形态：
liquid
- 保存条件：
Store at -20˚C
- 克隆性：
Polyclonal
- 适应物种：
Hu Ms Rat
- 保质期：
12 months
- 抗原来源：
Rabbit
- 供应商：
南京赛戈巍生物科技有限公司
- 宿主：
Rabbit
- 应用范围：
FC;IF
- 靶点：
Uniprot：P25942
- 抗体英文名：
CD40/TNFRSF5 Polyclonal Antibody PE-Cy7 Conjugated
- 规格：
50ul/100ul
计算分子量:30
配方:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
应用详情:Flow-Cyt=1:50-200 IF=1:50-200
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文献和实验T-cell dependent antigen specific in situ staining (Pe)
-conjugated secondary antibody (Typically SBA goat anti rabbit-AP 1:100, rabbit anti goat-AP 1:300) 22- wash twice 23- [optional] add a tertiary layer (rabbit anti-goat-AP on a goat anti-rabbit-AP) 24- wash thrice 25- develop AP (see protocol
Conjugation of monoclonal antibodies
derivatives, and Texas Red, may not work well for some IgM antibodies. Nonetheless, it is probably worth a try--we have had stunning success conjugating IgM antibodies with PE, APC, and Cy7APC. The conjugation of immunoglobulins is very straight-forward
Cell Surface Immunofluorescence Staining Protocol
antibody, resuspend cell pellet in residual buffer and add previously determined optimum concentrations of fluorochrome conjugated Streptavidin (SAv) reagent (e.g. SAv-PE, BioLegend Cat. # 405204) and incubate on ice for 15-20 minutes in the dark.
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