Rabbit anti-Horse IgG (H&L) pAb, AP conj.(bs-0308R-AP)-100ul/1ml产品图

Rabbit anti-Horse IgG (H&L) pA

b, AP conj.(bs-0308R-AP)-100ul/1ml
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  • ¥640 - 4800
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  • bs-0308R-AP
  • 2026年10月10日
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    • 文献和实验
    • 技术资料
    • 规格:

      100ul/1ml

    规格:100ul产品价格:¥640.0
    规格:1ml产品价格:¥4800.0
    产品编号bs-0308R-AP
    英文名称Rabbit Anti-Horse IgG H&L, AP conjugated
    中文名称碱性磷酸酶(AP)标记的兔抗马IgG H&L
    英文别名Rabbit Anti-Horse IgG H&L (AP); Immunoglobulin G;
    产品应用WB=1:1000-10000, IHC-P=1:100-500, IHC-F=1:100-1000, ELISA=1:1000-10000

    Not yet tested in other applications.
    Optimal working dilutions must be determined by the end user.

    交叉反应Horse
    抗体来源Rabbit
    免疫原Native Horse IgG
    亚型IgG
    性状Liquid
    纯化方法affinity purified by Protein A
    克隆类型Polyclonal
    浓度1.0 mg/ml
    储存液10 mM TBS (pH=7.4) with 1% BSA, 0.03% Proclin300 and 50% glycerol.
    保存条件Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
    注意事项This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
    背景资料Immunoglobulin G (IgG), is one of the most abundant proteins in serum with normal levels between 8-17 mg/mL in adult blood. IgG is important for our defence against microorganisms and the molecules are produced by B lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions; to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 1011 variants.

     

    应用推荐稀释比例
    {WB}{1:1000-10000}
    {IHC-P}{1:100-500}
    {IHC-F}{1:100-1000}
    {ELISA}{1:1000-10000}

     

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    图标文献和实验
    相关实验
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      IgG (Jackson ImmunoResearch, 111-096-006) Biotinylated anti-BrdU (AbCam, ab2284-125) Streptavidin-Alexa Flour 555 (Invitrogen/Molecular Probes, S21381) Goat serum Horse serum BSA 2N HCl DAPI Fluorescent mounting medium (Vectashield

    • Anti-P-CREB

      which employs goat anti-Rabbit HRP.12. Wash filter 3 times 7 minutes with 0.5% BSA in TBST.13. Perform Alkaline phosphatase reaction using NBT and BCIP substrates. I find that CREB usually comes up within several minutes of adding the AP substrates

    • Western Blot/Anti-P-CREB

      % nonfat dry milk works well for anti-PCREB, but in my hands milk is not as good as BSA from USB (it seems to mask the epitope).   Remove block solution and add primary antibody. For antiphosphoCREB (IgG, 0.7 mg/ml), I use 1:5,000

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    ¥640 - 4800