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- bs-0326Ms
- 2025年10月10日
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| 产品编号 | bs-0326Ms |
| 英文名称 | Mouse Anti-Bovine IgG H&L ascites |
| 中文名称 | 小鼠抗牛IgG H&L腹水 |
| 英文别名 | Bovine Immunoglobulin G; Bov IgG; |
| 交叉反应 | Bovine |
| 抗体来源 | Mouse |
| 免疫原 | Native Bovine IgG |
| 亚型 | IgG |
| 性状 | Lyophilized |
| 纯化方法 | Unpurified |
| 克隆类型 | Polyclonal |
| 保存条件 | Store at -20℃ stable for 2 years (lyophilized powder). Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | Immunoglobulin G (IgG), is one of the most abundant proteins in serum with normal levels between 8-17 mg/mL in adult blood. IgG is important for our defence against microorganisms and the molecules are produced by B lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions; to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 1011 variants. |
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文献和实验E L I S A w i t h p l a t e l e t s
for 1 hour with a 1 :1000 dilution of rat IgG2 a anti-mouse kappa chain monoclonal antibody conjugated to horse-radish peroxidase (LO-MK1 , LO/IMEX) reveal with o-phenylenediamine dihydrochloride (OPD) in revelation solution. SOLUTIONS
Purification of Antiserum or Ascites by Protein A/G Chromatography
A and protein G bind about 20 mg of IgG per ml of conjugated agarose. Therefore, the binding capacity of a 10 ml (5 ml) column is 200 mg (100 mg) of IgG. A high-titer rabbit antiserum has roughly 5 mg/ml of IgG, mouse ascites has roughly 10 mg/ml of IgG
the amount of antibody compared with other subclasses. In case of IgG1 subclass, add 3.3 M NaCl (192.85 g/L) to crude antibody preparation (serum, tissue culture supernatant, or ascites).Apply Binding buffer again ~ 200 ml.Apply Elution buffer ~ 100 ml.Collect 3 ml
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