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- 详细信息
- 技术资料
- 免疫原:
Recombinant human NUP50 protein
- 亚型:
IgG1
- 保存条件:
Store at -20°C. Avoid exposure to light. Stable for one year after shipment.
- 克隆性:
Monoclonal
- 标记物:
CoraLite® Plus 488 Fluorescent Dye
- 适应物种:
Human
- 宿主:
Mouse
- 应用范围:
FC (Intra)
- 靶点:
NUP50
- 抗体英文名:
CoraLite® Plus 488-conjugated NUP50 Monoclonal antibody
- 抗体名:
CoraLite® Plus 488-conjugated NUP50 Monoclonal antibody
- 规格:
100ul
经过测试的应用
| Positive FC (Intra) detected in | HL-60 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-67001 targets NUP50 in FC (Intra) applications and shows reactivity with Human samples.
| 经测试应用 | FC (Intra) |
| 经测试反应性 | Human |
| 免疫原 |
CatNo: Ag15144 Product name: Recombinant human NUP50 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 141-440 aa of BC028125 Sequence: DWIVKHVNTNPLCDLTPIFKDYEKYLANIEQQHGNSGRNSESESNKVAAETQSPSLFGSTKLQQESTFLFHGNKTEDTPDKKMEVASEKKTDPSSLGATSASFNFGKKVDSSVLGSLSSVPLTGFSFSPGNSSLFGKDTTQSKPVSSPFPTKPLEGQAEGDSGECKGGDEEENDEPPKVVVTEVKEEDAFYSKKCKLFYKKDNEFKEKGIGTLHLKPTANQKTQLLVRADTNLGNILLNVLIPPNMPCTRTGKNNVLIVCVPNPPIDEKNATMPVTMLIRVKTSEDADELHKILLEKKDA |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | nucleoporin 50kDa |
| 别名 | PRO1146, Nucleoporin Nup50, nucleoporin 50kDa, Nuclear pore-associated protein 60 kDa-like, 50 kDa nucleoporin |
| 计算分子量 | 468 aa, 50 kDa |
| 观测分子量 | 50-55 kDa |
| GenBank蛋白编号 | BC028125 |
| 基因名称 | NUP50 |
| Gene ID (NCBI) | 10762 |
| RRID | AB_3084296 |
| 偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
| 最大激发/发射波长 | 493 nm / 522 nm |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | Q9UKX7 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Nup50 (also known as Npap60) is a nucleoporin that binds directly to importin α and their interaction plays an important role in nuclear import. Nup50 is a mobile nucleoporin localized primarily in the nuclear basket of the nuclear pore complex (NPC) and in the nucleoplasm. Human Nup50 has two isoforms: Npap60L (1-469 aa), and its alternatively spliced isoform, Npap60S (29-469 aa) of Npap60L.
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