- ￥2800
- Proteintech
- CL647-80195
- 2025年10月09日
- IF/ICC, FC (Intra)
- Rabbit
- human
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- 详细信息
- 文献和实验
- 技术资料
- 亚型：
IgG
- 保存条件：
Store at -20°C. Avoid exposure to light. Stable for one year after shipment.
- 克隆性：
Recombinant
- 标记物：
CoraLite® Plus 647 Fluorescent Dye
- 适应物种：
human
- 宿主：
Rabbit
- 应用范围：
IF/ICC, FC (Intra)
- 靶点：
Phospho-P53 (Ser15)
- 抗体英文名：
CoraLite® Plus 647-conjugated Phospho-P53 (Ser15) Recombinant monoclonal antibody
- 抗体名：
CoraLite® Plus 647-conjugated Phospho-P53 (Ser15) Recombinant monoclonal antibody
- 规格：
100ul
经过测试的应用
| Positive IF/ICC detected in | etoposide treated HT-29 cells |
| Positive FC (Intra) detected in | UV treated A431 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.50 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL647-80195 targets Phospho-P53 (Ser15) in IF/ICC, FC (Intra) applications and shows reactivity with human samples.
| 经测试应用 | IF/ICC, FC (Intra) |
| 经测试反应性 | human |
| 免疫原 |
fusion protein |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | tumor protein p53 |
| 别名 | TP53, P53, Tumor suppressor p53, tumor protein p53, Antigen NY-CO-13 |
| 计算分子量 | 44 kDa |
| 观测分子量 | 53 kDa |
| GenBank蛋白编号 | BC003596 |
| 基因名称 | P53 |
| Gene ID (NCBI) | 7157 |
| RRID | AB_2935088 |
| 偶联类型 | CoraLite® Plus 647 Fluorescent Dye |
| 最大激发/发射波长 | 654 nm / 674 nm |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P04637 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
P53 is a 53 kDa protein that is activated in response to alteration of normal cell homeostasis, including DNA damage, nutrient starvation, heat shock, virus infection, pH change, hypoxia, and oncogene activation. P53 maintains genetic stability by regulating different processes, such as cell-cycle arrest, DNA synthesis and repair, programmed cell death, and energy metabolism. In non-stressed conditions these proteins bind p53, ubiquitylate it and target it for degradation by the proteasome. In stressed conditions the function of the MdM2-MdM4 complex is blocked by phosphorylation, protein-binding events and/or enhanced degradation. (PMID: 19935675, PMID: 24379683)
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文献和实验TBS 和 5% NGS 中封闭样品。市售的含有酪蛋白的封闭溶液与磷酸化的一抗结合后易减弱信号；因此，我们建议不使用含有酪蛋白的封闭剂进行磷酸化特异性抗体的检测。以上针对封闭步骤所提建议均是在以 SignalStain. Boost IHC Detection Reagent 作为检测试剂的情况下提出的。如果选择使用其他检测试剂，我们则建议使用与二抗来源相同的血清进行封闭。抗体：Phospho–Histone H2A.X (Ser139) (20E3) Rabbit mAb #9718样本：石蜡
-97KD)MTB087-120次国产80元分子量大小:（24、36、53、66、97KD）低范围蛋白质分子量标准（6.5-66KD）M3913-120次Sigma280元低范围蛋白质分子量标准（6.5-66KD）M3913-15×20次Sigma1198元分子量大小:（6.5、20、24、29、36、45、66KD）高范围蛋白质分子量标准（36-205KD)M3788-120次Sigma280元高范围蛋白质分子量标准（36-205KD)M3788-15×20次Sigma1198元
Incubating the membrane with primary antibody, which binds to the protein of interest Washing to remove any unbound primary antibody Incubating the membrane with a conjugated secondary antibody, which binds the first antibody Washing
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